植物药甘草中晶纤维的显微定量研究

A study of micro-quantitative methods for crystal fiber in herbs from glycyrrhiza uralensis

目的:改进植物药甘草中晶纤维显微定量的方法。方法:在已有显微定量方法的基础上,以混悬制样代替"水飞"制样,以血球计数板代替普通载玻片。利用线性分析方法确定取样量,用正交实验方法,优化制片用混悬液配比和定量需要观察制片的数量,进行混悬液稳定性考察,确定实验用时。结果:样品细度要求100目,样品定量需制片10张;混悬剂:由60%稀甘油和水合氯醛试液组成,比例19∶1;神木产甘草显微特征均数:1498±22个/mg。结论:引用血球计数板,提高显微定量的易操作性和准确性。

Objective：Improve the micro‐quantitative methods for crystal fiber in herbs fromglycyrrhiza uralen‐sis .Methods ：Based on existing micro‐quantitative methods ,they replace“Shuifei” sample‐making method （the process of which solid powder and liquid are grinded together in order to make suspension or remove contaminants） with Suspen‐ding sample‐making method ,and substitute blood‐counting plates for common microscope slides .Besides ,we determine sample quantity by linear analysis and optimize the proportion of suspension which is used for plates making as well as the number of sample plates for micro‐quantity with orthogonal experiment method .Through inspecting the suspension sta‐bility ,they could confirm the precise time period of the experiment .Results：The sample fineness should be 100 mesh . We need 10 slides to determine the sample quantity .Suspension：The mix of 60% glycerol and choral hydrate in the ratio of 19∶1 .The average number of micro‐characteristics of 1mg glycyrrhiza uralensis come from Shenmu is （1498 ± 22）/mg .Conclusion：It is a practical way to use blood‐counting plates to increase the ease of operation and accuracy of micro‐quantitative method .