碳青霉烯类抗生素亲水作用色谱方法研究及其应用

Analysis of carbapenems by hydrophilic interaction chromatography and its application

建立了测定人尿液和自来水中4种碳青霉烯类抗生素（比阿培南、美罗培南、多利培南和厄他培南）的亲水作用色谱方法，所用流动相具有较好的质谱兼容性，可用于色谱.质谱联用。以XAmide为色谱柱，考察了乙腈比例、缓冲盐浓度和pH值对4种抗生素保留的影响，提出了可能的保留机理；所发展的方法对目标样品具有良好的线性响应：比阿培南、多利培南和厄他培南的线性范围为0.1～250 mg／L， R^2＝0.9999；美罗培南的线性范围为0.5～250 mg／L， R^2＝0.9998；4种抗生素的定量限（ LOQ）为0.1～0.5 mg／L。尿液样品和自来水样品在5 mg／L和25 mg／L两个水平的加标回收率分别为100.4％～111.9％和79.6％～107.4％，相对标准偏差（ RSD）分别不大于1％和5％。该方法准确、灵敏、简便，可用于人尿液和自来水中多种碳青霉烯类抗生素的检测。

A hydrophilic interaction chromatographic （ HILIC ） method has been developed for the determination of the four carbapenems in human urine and tap water. The parameters inclu.ding acetonitrile amount, buffer concentration and pH on the retention behavior of the four car.bapenem antibiotics on an XAmide column were explored and the possible HILIC retention mechanism was proposed. Good linearities were obtained over the mass concentration ranges of 0.1-250 mg/L for biapenem, doripenem and ertapenem with correlation coefficients （ R^2 ）=0.999 9 and while it was 0.5-250 mg/L with R^2=0.999 8 for meropenem. The limits of quantifi.cation （ LOQs ） of all carbapenems were 0.1-0.5 mg/L. The spiked recoveries were within 100.4%-111.9% （ RSD〈1%） for urine samples and 79.6%-107.4% （ RSD〈5%） for tap water samples all at the spiked levels of 5 mg/L and 25 mg/L. The proposed method is accurate, sen.sitive, simple and suitable for the determination of the four carbapenems in human urine sam.ples and tap water samples.