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β-榄香烯对肺腺癌A549和H460细胞增殖和凋亡的影响 被引量:5

Effect ofβ-elemene on the proliferation and apoptosis of human lung cancer A549 and H460 cells
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摘要 目的:探讨β-榄香烯对肺腺癌A549和H460细胞增殖和凋亡的影响。方法:应用不同浓度的β-榄香烯干预A549和H460细胞后,台盼蓝拒染试验和MTT试验检测肿瘤细胞的增殖,流式细胞术检测A549细胞凋亡率,Western Blot法检测凋亡相关蛋白的表达。结果:不同浓度β-榄香烯能抑制肺腺癌A549和H460细胞的增殖,且呈剂量与时间依赖性,β-榄香烯干预后的A549细胞凋亡率明显升高,其相关抗凋亡蛋白Bcl-2和Bcl-xl表达下调。结论:β-榄香烯可抑制肺腺癌A549和H460细胞的增殖及诱导其凋亡,其作用机制可能与下调Bcl-2和Bcl-xl有关。 Objective:To investigate the effect ofβ-elemene on the proliferation and apoptosis of human Lung cancer cells.Methods:Lung cancer A549 and H460cells were treated with different concentrations ofβ-Elemene injection,The cell proliferation was measured by trypanblue stain and MTT.A549 apoptosis rate was measured by flow cytometry,the expression levels of apoptosis associated protein were explored by western blot.Results:The proliferation of human lung adenocarcinoma A549 and H460cell line were inhibited significantly by Elemene and the inhibition was time and dose-dependent.Theβ-Elemene could promote A549 apoptosis,and lower bcl-2and bcl-xl protein expression.Conclusion: β-Elemene can inhibit the proliferation and apoptosis by lower bcl-2and bcl-xl protein expression.
出处 《陕西医学杂志》 CAS 2015年第9期1107-1108,共2页 Shaanxi Medical Journal
基金 陕西省自然科学基金项目(2012JC2-06)
关键词 肺肿瘤/病理生理学 细胞凋亡 @β-榄香烯 Lung neoplasms/physiopathology Apoptosis @β-Elemene
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