基于转录组测序的波纹巴非蛤微卫星标记研究

Development of Microsatellite Markers from Transcriptome Data in Paphia undulata

应用MISA软件对波纹巴非蛤转录组测序数据进行分析,共获得7378个微卫星标记,从中选取95个位点设计引物,并在一个野生波纹巴非蛤群体中进行分析验证,结果显示：95个位点中能够扩增出清晰条带的位点有30个,其中15个位点表现为单态,15个位点呈现多态.使用15个多态位点进行波纹巴非蛤群体的遗传多样性分析,结果表明：这15个位点的等位基因数为2~9,平均等位基因数为4．733,平均有效等位基因数为2．438,平均观测杂合度和平均期望杂合度分别为0．270、0．516,平均多态信息含量为0．457.

Using MISA software, 7378 microsatellite markers were identified from the transcriptome data of Paphia undulata, in which 95 loci were chosen to design the primers and the PCR products were verified in a wild group of Paphia undulata. The results showed that a total of 30 loci were successfully amplified, of which 15 loci were monomorphic and other 15 loci were polymorphic. The allelic number （Na） and the effi-cient allelic number （Ne） in these 15 polymorphic loci were 4. 733 and 2. 438, respectively. The average ob-served heterozygosity （Ho） and the expected heterozygosity （He） were 0. 270 and 0. 516, respectively. The average of polymorphism information content （PIC） was 0. 457.