胎盘生长因子在胃癌组织中的表达及其临床意义

Expression and Clinical Significance of Placental Growth Factor in Gastric Cancer Tissue

目的探讨胎盘生长因子（P1GF）与胃癌发生和发展的关系。方法回顾性收集2013年3月至2014年12月期间在绵阳四。四医院胃肠外科行胃癌根治术的88例胃癌患者作为研究对象，取其肿瘤组织（胃癌组）及癌旁组织（癌旁组织组）行免疫组织化学染色和逆转录-聚合酶链反应法（RT-PCR）检测P1GFmRNA及其蛋白的表达，取其外周血行酶联免疫吸附法（ELISA）检测P1GF的浓度。收集2014年9月于绵阳四。四医院体检科行体检的30例健康体检者（体检组）的外周血行ELISA检测P1GF的浓度。比较胃癌组和癌旁组织组组织中P1GFmRNA及其蛋白的表达水平，比较胃癌组患者和体检组人员外周血中P1GF的浓度，并分析胃癌组患者癌组织中P1GFmRNA及其蛋白表达、外周血中P1GF浓度与其临床病理学特征的关系。结果RT-PCR结果显示：胃癌组组织中P1GFmRNA的表达水平为0．569±0．166，高于癌旁组织的0．037±0．020（t=-29．948，P=0．000）。免疫组织化学染色结果显示：胃癌组组织中P1GF的表达阳性率为80．7％（71／88），高于癌旁组织组的5．7％（5／88），差异具有统计学意义（x^22=100．867，P=0．000）。ELISA法检测结果显示：胃癌组患者外周血中的P1GF浓度为（57．247±9．800）ng／L，高于体检组的（10．351±1．715）ng／L，差异有统计学意义（t=43．000，P=0．000）。胃癌患者癌组织中P1GFmRNA及其蛋白的表达与肿瘤直径、pT分期、pN分期、分化程度及Borrmann分型均有关（P〈0．050），肿瘤直径〉4cm、pT3-4期、pN3期、低分化及Borrmann分型为Ⅲ-Ⅳ型者P1GFmRNA的表达水平和P1GF的表达阳性率均较高；而PIGFmRNA及其蛋白的表达与年龄、性别、pM分期及胃肠型均无关（P〉0．050）。胃癌患者外周血中P1GF的浓度与年龄、性别、肿瘤直径、pT分期、pN分期、pM分期、分化程度、Borrmann分型及胃肠型均无关（P〉0．050）。结论胃癌患者癌组织及血液中PIGF的表达异常可能与胃癌的发生和发展有关。

Objective To investigate the relationship between placental growth factor （P1GF） and the gastric cancer. Methods The cancer tissues （cancer tissue group） and para-cancer tissues （para-cancer tissue group） of 88 patients with gastric cancer who underwent surgery in Sichuan Mianyang 404 Hospital from Mar. 2013 to Dec. 2014 were collected retrospectively, to determine the expressions of PlGF mRNA and its protein by polymerase chain reaction （PCR） and immunohistochemistry method. In addition, blood samples of 30 normal persons （normal person group） who got examina- tion in Sichuan Mianyang 404 Hospital in Sep. 2014 and 88 patients with gastric cancer （gastric cancer group） were collected to detect the concentration of serum P1GF, by using enzyme linked immunosorbent assay （ELISA）. Comparison of the expressions of P1GF mRNA and its protein between cancer tissue group and para-cancer tissue group, concentration of PlGF between cancer tissue group and normal person group were performed, as well as the relationship between expressions of serum P1GF mRNA/P1GF in gastric cancer tissues and dinicopathological features of patients with gastric cancer, and relationship between concentration of P1GF in blood and clinicopathological features of patients with gastric cancer was explored by univariate analysis. Results The expression level of P1GF mRNA （0.569±0.166 vs. 0.037±0.020, t=-29.948,P=0.000） and positive-expression rate of P1GF ~80.7% （71/88） vs. 5.7% （5/88）, x^2=100.867, P=0.000] were significantly higher in cancer tissue group than those of para-cancer tissue group. And the concentration of P1GF in blood of patients in gastric cancer group was higher than that of normal person group [ （57.247±9.800） ng/L vs. （10.351±1.715） ng/L, t=43.000, P=0.0003. The expressions of P1GF mRNA and its protein were both correlated with diameter of tumor, pT staging, pN staging, differentiation, and Borrmann type （P〈0.050）. The expression levels of P1GF mRNA and its protein in that patients with diameter of tumor greater than 4 cm, pT3-4 staging, pN3 staging, low differentiation, and Borrmann III - IV staging were higher. While there were no significant correlation between expressions of P1GF mRNA/protein and age, gender, pM staging, and gastrointestinal type （P〉0.050）. Concentration of serum P1GF of gastric cancer patient wasn＇t significantly correlated with age, gender, diameter of tumor, pT staging, pN staging, pM staging, differentiation, Borrmann type, and gastrointestinal type （P〉0.050）. Condnsion The abnormal expression of P1GF at gastric cancer tissues may play an important role in pathogenesis of gastric cancer.