摘要
为了提高德氏乳杆菌保加利亚亚种NCFB2772(Lactobacillus delbrueckii subsp.bulgaricus NCFB2772,L.burlgaricus NCFB2772)胞外多糖(EPS)的产量,在该菌株高产EPS发酵条件优化前期研究的基础上,进一步从基础培养基的筛选、碳氮源的优化和促生长因子三方面对该菌株高产EPS的营养条件进行优化。结果表明:在筛选的基础培养基MRS2中添加20 g/L的果糖、10 g/L的蛋白胨(进一步优化二者之比为1.5∶1.0)、6%的苹果汁,L.burlgaricus NCFB2772产EPS的量(854.5 mg/L)和活菌数(未显示)都达到最高,比优化前EPS的产量(460 mg/L)提高了近1倍。
To improve the extraceUular polysaccharides （EPS）yield of LactobaciUus delbrueckii subsp, bulgaricus NCFB2772 （L. burlgaricus NCFB2772）, the nutritional conditions of high - yield. EPS of the strain were optimized further through three aspects including the screening of the basic medium, optimization of carbon and nitrogen sources and growth - promoting factors based on the optimization of fermentation conditions for the high - yielding EPS of the strain in author＇ s earlier study. The results showed that the EPS yield and viable count of L. burlgaricus NCFB2772 could reach the maximum when adding 20 g/L of fructose and 10 g/L of peptone （further optimized the ratio of 1.5：1.0 between them）, and 6% of apple juice in the screened basic medium MRS2, while the EPS yield （854.5 mg/L） after the optimization increased nearly 1 times more than the EPS yield （460 mg/L） before the optimization
出处
《黑龙江畜牧兽医》
CAS
北大核心
2015年第9期42-45,49,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金面上基金项目(30771524)
