1株野生茶树菇的鉴定及其母种培养条件研究

Study on Identification and Mycelial Culture Condition of a Wild Agrocybe aegerita Strain

为更好地开发利用茶树菇,同时丰富国内茶树菇种质资源,以采自贵州的1株野生茶树菇NC1菌株为材料,对其进行内转录间隔区ITS（internal transcribed spacer）鉴定和母种培养条件研究,包括对菌丝生长的最适碳源、氮源、C/N比、最适温度、最适p H等。结果表明：该菌株为柱状田头菇（Agrocybe aegerita）,其菌丝生长的最适碳源为玉米淀粉,其次为葡萄糖或果糖,最适氮源为酵母粉,其次为蛋白胨,最适C/N比为30/1~40/1,最适温度为29℃,最适p H 5.0~6.0。研究结果可为野生茶树菇NC1菌株进一步驯化栽培和开发利用提供参考。

In order to development the utilization of Agrocybe aegerita and enrich the germplasm resources of Agrocybe aegerita in China, a wild Agrocybe strain NC1 isolated from Guizhou was used as materials. Strainidentification by internal transcribed spacer（ITS） molecular biological techniques and culture condition ofmother mycelial were studied. The biological characteristics of mycelial growth on different carbon source,nitrogen source, C/N ratio, temperature, p H value were studied. The results showed that the wild strain wasidentified as Agrocybe aegerita NC1. The most suitable carbon source was the corn starch, followed by glucoseor fructose. And the optimal nitrogen source was yeast powder, followed by peptone. The optimum C/N ratiowas 30/1-40/1, the optimum temperature was 29℃, and the optimal p H was 5.0-6.0. The results of this studymay provide a theoretical basis for the domesticated cultivation and development and utilization of wild Agrocybe aegerita strain NC1.