P2X2受体在匹罗卡品致慢性癫痫发作大鼠海马组织中表达增高

Increased Expression of P2X2 Receptor in Hippocampus of Rats with Pilocarpine-induced Spontaneous Recurrent Seizure

目的通过研究匹罗卡品致癫痫大鼠海马组织中P2X2受体(嘌呤受体)的表达变化,探讨其在颞叶癫痫发病中的作用机制。方法应用氯化锂-匹罗卡品腹腔注射建立大鼠颞叶癫痫模型,并予以P2X2受体拮抗剂亮蓝G(Brilliant Blue G,BBG)腹腔注射,应用Western blot及Real-time PCR技术检测大鼠海马组织P2X2受体的表达,免疫组化技术检测各组大鼠海马组织中谷氨酸(glutamate,GLU)表达水平。结果 Western blot及Real-time PCR结果显示:慢性自发性癫痫发作组中P2X2表达明显增高(P<0.05),BBG干预组表达减低P<0.05)。免疫组化检测显示:GLU在BBG干预组较慢性自发性发作组中释放减少(P<0.05),且与BBG剂量呈负相关性。结论 P2X2受体通路可能参与颞叶癫痫的发病过程,并有望成为新一代治疗颞叶癫痫的药物靶点。

Objective To investigate expression of P2X2 receptor in lithium-pilocarpine-treated rats and the possible role it played in temporal lobe epileptis（TLE）.Methods TLE rat model was established by lithium-pilocarpine intraperitoneal injection.Then,brilliant blue G（BBG）,a specific P2X2 receptor antagonist was injected intraperitoneally to the TLE rats.The expression of P2X2 receptor and P2X2 receptor mRNA in hippocampus was detected by Western blotting and RT-qPCR respectively,while the level of glutamate（GLU）in hippocampus was assessed by immunohistochemistry.Results The expression of P2X2 receptor in spontaneous recurrent seizure group was significantly increased（P〈0.05）,while the expression of P2X2 receptor in the rats treated with BBG was significantly decreased（P〈0.05）,concomitantly,aparallel result was investigated in the expression of P2X2 receptor mRNA（P〈0.05）.Furthermore,GLU was also significantly reduced in the BBG group as compared with spontaneous recurrent seizure group（P〈0.05）,and it was negatively correlated with the dose of BBG.ConclusionP2X2 receptor may play a critical role in the development of TLE,and could be a potential therapeutic target for the treatment of TLE.