响应面分析法优化平菇液体菌种培养基的研究

Study on optimization of liquid seed culture medium of Pleurotus ostreatus by response surface methodology

以平菇为研究对象,用菌丝体干重作为考查目标,筛选平菇液体菌种培养基.在单因素实验的基础上,利用Plackett-Burman实验确定影响平菇菌丝体干重的关键因素.以玉米粉、麸皮和KH2PO4为自变量,菌丝体干重为响应值,根据Box-Behnken中心组合设计原理,研究各自变量及交互作用对菌丝体干重的影响,得出平菇液体菌种最适培养基配比:玉米粉5.00%、麸皮4.50%、KH2PO40.25%.在此条件下,菌丝体干重实测可达9.640g/L,与理论预测值9.752g/L相比,其相对误差为1.15%.说明响应面预测模型具有一定的可靠性.

Pleurotus ostreatus as the research object, with the dry weigh of mycelium as a measure indicator, three parameters including corn flour, wheat bran and KH2 PO4 were optimized using the Plackett - Burman design and Box - Be- hnken certral composite design based on single factor investigations for optimizing the components of liquid strain culture medium of Pleurotus ostreatus . The results showed that the optimal medium were corn flour 5.00%, wheat bran 4.50%, KH2PO40.25%. Under the conditions, the dry weight of mycelia reached 9.640 g/L, compared with the theoretical predicted value of 9.752 g/L, the relative error was 1.15%. It was indicated that the reliability of the model was proved fully.