摘要
目的探讨儿童非典型呼吸道病原体感染与血清总IgE水平的相关性。方法应用呼吸道九联检检测试剂盒(间接免疫荧光法)对1 913例儿童呼吸道感染患者的血清样本进行9项非典型呼吸道病原体的IgM抗体检测,包括肺炎支原体(MP)、嗜肺军团菌(Lp)、Q热立克次体(QFR)、肺炎衣原体(CPn)、腺病毒(Adv)、呼吸道合胞病毒(RSV)、甲型流感病毒(IAv)、乙型流感病毒(IBv)和副流感病毒(PIV)1、2和3型;同时应用免疫散射比浊法检测这些血清样本的总IgE水平。对所得数据进行统计学分析。结果 1 913例儿童呼吸道感染患者中检出有非典型病原体感染者991例(阳性组),无非典型病原体感染者922例(阴性组);阳性组以MP及MP合并其他病毒感染者为主,共650例(占65.59%)。阳性组患者血清总IgE水平明显高于对照组,且MP感染患儿血清总IgE水平高于IBv、Adv及RSV感染者。在总IgE水平高于临床参考范围上限(100kU/mL)的患者中,单纯MP感染者占31.29%,明显高于IgE低水平组(≤100kU/mL)中的比例(21.30%);而单纯RSV感染者占1.88%,明显低于IgE低水平组中的比例(6.53%);单纯Adv感染者占3.13%,明显低于低水平组中的比例(6.53%)。结论在儿童非典型呼吸道病原体感染中以MP感染最常见;MP感染者较非MP感染者血清总IgE水平更高。
Objective To investigate the correlation between atypical respiratory pathogens infection and serum total IgE levels . Methods Serum IgM level was detected in 1 913 blood samples of children with atypical respiratory infection by using indirect im‐munofluorescence assay ,including mycoplasma pneumonia(MP) ,legionella pneumophila (LP) ,rickettsia Q(QFR) ,chlamydia pneu‐monia(CPn) ,adenovirus (Adv) ,respiratory syncytial virus (RSV) ,influenza A virus (IAv) ,influenza B virus (IBv) and parainflu‐enza virus (PIV)1/2/3 .The serum total IgE level was detected by immune scatter turbidimetry .Software SPSS 17 .0 was used in data statistical analysis .Results A total of 991 out of 1 913 samples of respiratory inflected children exhibited positive(positive group) ,while 922 exhibited negative(negative group) in indirect immunofluorescence assay .650 out of the 991 positive samples (65 .59% ) contained MP infection and the combination of MP infection and other virus infections .The serum total IgE level in posi‐tive group was significantly higher than that of the negative group ,and the serum total IgE level in samples with MP infection was higher than that in samples with IBv infection ,Adv infection ,and RSV infection .In the samples in which serum total IgE level was higher than the clinical reference range (100 kU/mL) ,the infection rate of MP infection alone was 31 .29% ,which was evidently higher than that in samples of low IgE level(〈 100 kU/mL ,21 .30% ) .On the other hand ,the infection rate of RSV alone was 1 .88% and the infection rate of Adv alone was 3 .13% ,which were both evidently lower than those in samples with normal serum total IgE level(both 6 .53% ) .Conclusion MP is the most common pathogen in children with atypical respiratory pathogen infec‐tion ,and can lead to higher serum total IgE levels .
出处
《国际检验医学杂志》
CAS
2015年第17期2480-2481,2484,共3页
International Journal of Laboratory Medicine
基金
生物医药领域产学研医合作研究项目(14DZ1940200)