TRPV1在一过性肠道感染大鼠模型内脏高敏感形成中的意义

Role of TRPV1on the rats with visceral hypersensitivity induced by transient intestinal infection

[目的]研究TRPV1在SD大鼠一过性肠道感染旋毛虫后形成内脏高敏感中的作用。[方法]30只大鼠随机分为正常对照组、假手术组和内脏高敏感组。内脏高敏感组给予旋毛虫感染,假手术组给予同等量的PBS灌胃,正常对照组不作任何处理。8周后,观察各组结直肠不同扩张压力下(20mmHg、40mmHg、60mmHg、80mmHg)腹壁肌电活动变化;采用Western-blot检测远端结肠TRPV1蛋白质水平的表达。[结果]1正常对照组、假手术组和内脏高敏感组结直肠不同扩张压力与腹壁肌电活动之间有显著相关性,各组相关系数与P值分别为:正常对照组r=0.825、P=0.000,假手术组r=0.831、P=0.000,内脏高敏感组r=0.625、P=0.006。在20mmHg、40mmHg和60mmHg扩张压力下,3组间AUC均差异有统计学意义(P<0.05);在80mmHg,3组间AUC差异无统计学意义(P>0.05)。2正常对照组、假手术组、内脏高敏感组TRPV1光密度相对值分别为0.95±0.17、0.94±0.24、1.57±0.31,内脏高敏感组较正常对照组、假手术组均显著升高(P<0.05)。[结论]内脏高敏感的形成与TRPV1高表达密切相关。

[Objective]To investigate the role of TRPV1 on the formation of visceral hypersensitivity on rats induced by transient intestinal infection.[Methods]Thirty male Sprague-Dawley rats were randomly divided into normal control,sham operation and visceral hypersensitivity.The rats in visceral hypersensitivity were infected by administering 1.0ml of PBS containing 8 000 T.spiralis larvae by gavage.Those rats in sham operation were only administered 1.0ml of PBS.Electromyography（EMG）was recorded at8 th week after adaptive feeding for evaluating visceral sensitivity.The areas under curve（AUC）of EMG in 20 swere calculated to evaluate the visceral sensitivity.Western-blot was respectively employed to detect protein TRPV1 expression.[Results]1 Under different grades levels of CRD pressure,AUCs showed significant correlation in the three groups respectively（P0.05）.The coefficient of correlation and P value of the three groups were 0.825,P=0.000;0.831,0.000;0.625,0.006 respectively.Under 20 mmHg,40mmHg and 60 mmHg,the difference of the AUC among the three groups was significant（P=0.034,0.043,0.048respectively）.Under 80 mmHg,no significant difference was found in three groups（P0.05）.2In normal control,sham operation and visceral hypersensitivity group,the IODs of TRPV1 protein were 0.95±0.17,0.937±0.24,1.57±0.31 respectively.The expression of TRPV1 of visceral hypersensitivity was significantly increased than sham operation and normal control（P0.05）.[Conclusion]The increased expression of TRPV1 plays an important role in the formation of visceral hypersensitivity.