hsa-miR-10b在3株宫颈癌细胞系中的表达及靶基因预测

Expression of hsa-miR-10b in three cervical cancer cell lines and its target genes

目的分析3株宫颈癌细胞系中hsa-miR-10b表达水平,并预测hsa-miR-10b的靶基因。方法用PCR技术检测3株宫颈癌细胞系中hsa-miR-10b表达水平;Pub Med检索hsa-miR-10b相关文献;miRbase和NCBI分析其序列保守性及所在基因组特征;TargetScan、PicTar及miRanda数据库预测其靶基因,并结合已证实的靶基因对其进行功能和信号通路富集分析。结果与C-33A相比,HeLa和CaSki中hsa-miR-10b表达水平显著下调(P<0.01);hsa-miR-10b与多种肿瘤的发生发展有关;hsa-miR-10b定位于人染色体2q31.1,在各物种之间具有高度保守性;其靶基因主要参与转录、基因表达调控和细胞增殖等生物学过程(P<0.05),涉及肿瘤、细胞周期和ARF等信号通路(P<0.05)。结论 hsa-miR-10b功能广泛,与宫颈癌的发生发展密切相关,靶基因的预测为后续研究提供依据。

Objective To analyze the expression of hsa-miR-10b in three cervical cancer cell lines, and to find the target genes of hsa-miR-10b. Methods PCR was applied to measure expression levels of hsa-miR-10b in C-33A, HeLa and CaSki. The relative studies on hsa-miR-10b were retrieved from PubMed. The sequence and genome char- acteristics of hsa-miR- 10b were analyzed on line by miRbase and NCBI. The target genes of hsa-miR- 10b were searched by TargetScan, PicTar and miRanda, and then demonstrated by Gene Ontology and Pathway Enrichment analysis. Results Compared with C-33A, the expression of hsa-miR- 10b significantly reduced in HeLa and Caski （ P 〈 0. 01 ）. Current studies showed that hsa-miR- 10b was related with multiple tumorigenesis, hsa-miR- 10b was lo- cated in human chromosome 2q31.1 and highly conserved among different species. The target genes were enriched in the biological processes of transcription, gene expression regulation, cell proliferation and etc. （P 〈 0. 05 ）. Pathway analysis showed that these target genes were responsible for biochemical erents mediated by to the signaling pathways of cancer, cell cycle, ARF and etc. （P 〈 0. 05 ）. closely related with the occurrence and development cal basis for the further study. Conclusions hsa-miR-lOb may have extensive functions, and in cervical cancer. Prediction of target genes provides a theoreti-