大豆根腐病生防细菌优势菌株的筛选、鉴定及生防效果验证

Screening and Identification of Biocontrol Dominant Bacterium against to Soybean Root Rot

根腐病是影响大豆产量的主要病害之一,镰刀菌(Fusarium sp.)是引起我国大豆根腐病的主要病原菌。以致病尖孢镰刀菌为研究对象,通过平板对峙法从实验室保存的237株芽孢杆菌筛选到一株对该尖孢镰刀菌具有明显抑菌活性的芽孢杆菌8-32。对芽孢杆菌8-32经菌落、菌体形态,16S rRNA基因序列及生理生化性质鉴定,该菌株为枯草芽孢杆菌(Bacillus subtilis)。温室盆栽结果表明:该生防菌对大豆根腐病具有很好的防治效果,其病情指数减少32.08%。

Root rot is one of the most serious diseases which caused reduction of soybean yield,and Fusarium. sp is considered as the main pathogen fungi caused this disease in China. In this study,Fusarium oxysporum was taken as indicative fungus,we screened one strain 8-32 against to Fusarium oxysporum from 237 strains saved in our laboratory using the method of dual culture on agar plate. According to colony morphology,16 S rRNA gene sequence,physiological and biochemical characters,we identified this strain as Bacillus subtilis. Results showed that the strain 8-32 reduced the incidence of soybean root rot disease index under controlled glasshouse conditions,which up to 32. 08%. This strain,8-32,has the potential to form production to control the soybean root rot.