大豆疫霉根腐病相关miRNA的鉴定

Identification of miRNA Resistant to Phytophthora Root Rot in Soybean

microRNA(miRNA)是近年来在真核生物中发现的一类具有调控功能的非编码RNA,通过碱基互补在基因转录和转录后水平调控靶基因的表达,参与调控植物生长发育,并在响应多种生物及非生物胁迫中发挥重要作用。本文利用大豆疫霉菌2号生理小种(P6497)接种8个栽培大豆品种,通过Northern blotting技术,对大豆中的7个保守miRNAs进行检测。结果发现,有6个miRNAs在这8个大豆栽培品种中都有表达,但在接种病原菌前后没有明显的差异。使用高通量的小分子RNA芯片技术检测差异表达的miRNAs。利用抗性品种Williams 82和感病品种Williams分别接种P6497,获得部分与大豆疫霉菌侵染相关的miRNAs。结果显示,部分miRNAs可能参与调控大豆疫霉根腐病抗性。

The microRNAs（ miRNAs）,a type of short（ 20-24 nucleotides）,non-coding RNA molecules,mediate repressive gene expression through RNA silencing at the transcriptional and post-transcriptional level,and play an important role in the regulation of plant growth and development,and defense and response to various abiotic and biotic stresses. Phytophthora root and stem rot（ PRR） of soybean caused by Phytophthora sojae is a destructive disease affecting soybean production regions throughout the world. However,the miRNAs-regulation networks and function in response to PRR are not clear in Glycine max. In this paper,seven conserved miRNAs were detected in eight cultivars of pre- and post-inoculation by Northern blotting. The results showed that,six conserved miRNAs were expressed in eight cultivars in pre- and post-inoculation,but there is no significant difference in pre- and post-inoculation. Furthermore,we used microarray to detect miRNA expression pattern.The result showed that some miRNAs play very important roles in response to PRR in Glycine max.