熊胆眼药水对兔眼刺激性的实验研究

Stimulating test of bear bile eye drops on rabbit eyes

目的 探讨熊胆眼药水在频繁应用时对眼部的刺激反应,为药品使用的安全性提供实验依据. 方法 将熊胆眼药水以不同的频率（分别间隔1,5,10 min）滴入家兔右眼结膜囊,使用0. 9%氯化钠溶液滴入同一只家兔的左眼作为对照,每次均滴入1滴,连续滴入10次. 于最后1次滴药后0,6,24, 48 h,观察家兔的眼部刺激反应. 结果 间隔5,10 min组,家兔右眼眼结膜无充血、水肿、分泌物,虹膜正常,也无角膜混浊,根据眼刺激反应评分标准,其评分为1分,属无刺激. 间隔1 min组家兔右眼在实验完毕后观察,显示结膜轻度充血,轻度水肿,结膜囊分泌物增多,评分平均为5分,属轻度刺激;在6h观察时评分下降为平均3分,属无刺激性;12 h后评分平均值下降为1分以下. 结论 熊胆眼药水在高频率使用时可对兔眼造成轻度刺激,其刺激反应可逆,总体安全性较高,但应避免高频率使用.

Objective To explore the stimulating effect of bear bile eye drops on rabbit eyes frequently in order to provide experimental basis for drug use. Methods Bear bile eye drops dripped into the right eye of rabbit conjunctival sac as experimental group （ different frequency as 1, 5, 10 min） while normal saline was used in the left eye of rabbit as the control group （ one drop once and 10 times continually） . We observed the stimulation of rabbit eyes at 0, 6, 12, 24, 48 hours after the last dripping. Results At the observation of 5 and 10 min interval set, the right eyes of rabbit had no hyperemia, no edema, no secretion, no maculae corneae, and normal iris that was 1 score according to eye stimulation response scale belonging no irritation. After 1 min interval set observation of right eyes, they presented light conjunctiva hyperemia, light edema, increased secretion of conjunctiva sac with 5 score belonging to light stimulation;but it decreased to 3 scores at 6 h interval set belonging to no stimulation;the average score declined underneath 1 score at the observation of 12 h interval set. Conclusions Bear bile eye drops can arouse reversible light stimulation when used in high frequency, so the safety is high, but we should avoid the high frequency of use.