摘要
本研究通过免疫荧光分析,鉴定NTERA-2细胞是否具有精原干细胞特征。研究结果显示,重要精原干细胞标记分子GFRα-1和DDX4在NTERA-2细胞中均表现为阳性表达,NTERA-2细胞具有精原干细胞的特征。初步探讨了重要细胞信号转换蛋白JNK特异性抑制剂SP600125对NTERA-2细胞生长和增殖的影响,为进一步以NTERA-2细胞为精原干细胞模型开展JNK对生殖细胞发育调节功能的研究奠定基础。
This study was to identify the characteristics of NTERA-2 cells to find whether out has it the characteristics of spermatogonial biology. Our results showed that NTERA-2 cells expressed some markers of spermatogonial stem cells, such as GFRct-1 and DDX4. It suggest that NTERA-2 ceils maybe useful as a model for studying spermatogonial stem cell proliferation and differentiation in vitro. With the treatment by SP600125, the proliferation of NTERA - 2 cells is se- riously inhibited. The results are the foundation of further research on the function of JNK in germ cell development.
出处
《激光生物学报》
CAS
2015年第4期364-367,共4页
Acta Laser Biology Sinica
基金
国家自然科学基金(31172399)资助项目
湖南省教育厅项目资助(15C1274)
湖南省研究生科研创新项目资助(CX2013B212)