丹参愈伤组织培养中激素浓度的优化研究

Study on Optimization of Hormone Concentration in Callus Culture of Saliae Miltiorrhiza Bge

目的:优化丹参愈伤组织继代培养中激素浓度的最佳组合。方法:以丹参种子为外植体,以无菌苗下胚轴诱导的初代愈伤组织为材料,以愈伤组织净增鲜重为指标,利用二次正交旋转组合设计方法进行数学模拟,建立数学模型,利用响应面法进行激素浓度组合寻优。结果:丹参愈伤组织继代培养中激素浓度的最佳组合为6-BA 1.514 mg/L+NAA 2.059 mg/L+2,4-D 2.137 mg/L;愈伤组织的净增殖鲜重为35.66 g。

Objective： To seek optimum combination of hormone concentration during callus subculture of Glycyrrhiza uralensis. Methods： The seed of Saliae Miltiorrhiza Bge as explants,first generation callus induced by embryo root and hypocotyledonary axis of sterilized seed as material,the net fresh weight of callus as indicator,mathematical model were established by quadratic orthogonal rotary composite experimental design. The concentration of hormones were optimized by method of response surface. Result： The optimum combination of hormone concentration during callus subculture of Saliae Miltiorrhiza Bge is 6-BA 1. 514 mg / L,NAA 2. 059 mg / L,2,4-D 2. 137 mg / L. The net fresh weight of callus is35. 66 g.