响应面法优化芸豆蛋白提取工艺

Optimization of Protein Extraction from Phaseolus vulgaris by Response Surface Methodology

为探讨芸豆蛋白提取的最佳工艺条件,在单因素试验的基础上,根据Box-Behnken的中心组合试验设计原理,设计4因素3水平试验,研究了p H、提取时间、液料比和提取温度对芸豆蛋白提取率的影响。结果表明,在芸豆蛋白提取工艺参数为p H 10.4、提取时间1.5 h、液料比20︰1(m L/g)、提取温度40℃时,测得芸豆蛋白提取率为68.55%。

To optimize the extraction condition for protein from Phaseolus vulgar＆, on the basis of single-factors experiments,according to Box-Behnken central composite experimental design principles, design four factors and three levels test. The extraction efficiency of protein was investigated with respect to four variables including pH, time, solvent/solid ratio, temperature. The results indicated that the optimum conditions of extraction were determined as 10.4, 1.5 h, 20 ： 1 （mL/g）, 40 ~C and for pH, time, liquid-solid ratio and temperature respectively, under the conditions, the extraction rate was 68.55%.