牦牛气管中硫酸软骨素提取工艺研究

Study on Extraction Process of Chondroitin Sulfate from Yak Trachea

以牦牛气管为原料,采用超声波辅助碱处理提取硫酸软骨素,研究了料液比、超声波功率、超声时间对其得率的影响,通过正交试验优化了提取工艺。结果表明,超声波辅助碱提取牦牛气管中硫酸软骨素的工艺条件为:料液比1∶6(g/m L),超声波功率为550 W,超声时间为30 min,在提取温度35℃,p H 12的条件下硫酸软骨素的得率可达18.97%。各因素对硫酸软骨素得率的影响顺序依次为:超声功率>超声时间>料液比。比较了TCA法、Sevage法、胰蛋白酶法的除蛋白效果,其中胰蛋白酶法效果最佳,蛋白质去除率可达85.78%,硫酸软骨素纯度达85.56%。

Use ultrasonic wave to extract chondroitin sulfate fi＇om yak trachea, research on the effect of solid-liquid ratio, ultrasonic power and ultrasonic treatment time on chondroitin sulfate the yield. The technological optimum parameters were found as follows： solid-liquid ratio was 1 ： 6 （g/mL）, ultrasonic power was 550 W, ultrasonic treatment time was 30 min, and extraction temperature was 35 ~C, pH 12, under these conditions, chondroitin sulfate the yield could reach 18.97%. The primary and secondary factors which affected the yield showed as following： ultrasonic power〉ultrasonic treatment time〉 solid-liquid ratio. Three deproteinization methods： trichloroacetic acid method, Sevage method and parenzyme method were compared. It showed that the parenzyme method was the best, its protein removal rate could reach 85.78%, and purity of chondroitin sulfate was 85.5%A.