一株稳产麦角新碱菌株的分离鉴定及生物学特性研究

Isolation and identification of an ergonovine stable yield strain and its biological characteristics

目的对实验室筛选到的一株产麦角新碱的菌株F33进行分离鉴定,并对该菌株的产碱稳定性和发酵条件进行研究。方法采用24孔板高通量筛选方法从麦角菌核中筛选菌株F33,通过质谱和核磁共振氢谱对菌株F33发酵获得的代谢产物进行结构鉴定,利用形态特征、26S r DNA序列和ITS序列同源性比对分析初步鉴定菌株F33,并对该菌株的生物学特性进行研究。结果菌株F33为Claviceps paspali(雀稗麦角菌),遗传稳定性较好,其主要代谢产物为麦角新碱,对发酵条件初步优化菌株产碱量由100μg/m L增加到600μg/m L,提高了约6倍。结论菌株F33是一株具有开发价值的菌株。

Objective To isolate and identificate a ergonovine production strain F33 that conserved in our laboratory, and study on the stability of ergot alkaloids production and the fermentation conditions. Methods Strain F33 was obtained through 24-well formats high-through screening in sclerotia. The metabolite from the cultural of strain F33 was determined by ESI-MS and 1H-NMR. The strain F33 was preliminarily identified by morphological characteristics combined with 26S rDNA gene sequences and ITS gene sequences. Besides, further study on the biological characteristics of strain F33 was carried out. Results The strain F33 was certified as Claviceps paspali with good genetic stability and the main metabolite was identified as ergonovine. The fermentation conditions of strain F33 were preliminarily optimized and the ergot alkaloids production was increased by 6 times, from the initial titers 100μg/mL to 600μg/mL. Conclusion The strain F33 is a promising strain.