均匀设计和抗心肌缺血效应的人参健心胶囊组方优化研究

Formula Optimization in Renshen Jianxin Capsule Based on Uniform Design and Anti-myocardial Ischemia Effect

目的 筛选人参健心胶囊抗心肌缺血主效应中药并优化其最佳剂量水平,实现人参健心胶囊的二次组方优化。方法 遵循“均匀设计—药效测定—数学建模—组方优化”的思路,按照U_（10）（10~8）均匀设计方案,以人参健心胶囊所含的8味中药为考察因素,异丙肾上腺素诱导的大鼠心肌缺血性心电图变化为药效学指标,采用逐步回归分析构建药物因素与药效学指标间的数学模型,筛选抗心肌缺血主效应中药,用网格算法优选抗心肌缺血主效应中药的最佳剂量水平,寻找优选中药的最佳配伍组合。结果 逐步回归分析建立的回归方程为y=1.7889-0.3247人参×丹参-0.0663黄芪×泽泻,纳入的预测因素有人参、丹参、黄芪和泽泻,网格算法优化得到的最佳配比剂量为人参1.62 g、黄芪4.62 g、丹参2.43 g及泽泻1.66 g。结论 应用均匀设计结合逐步回归分析和网格算法可较好地实现中药复方的二次组方优化。

Objective To realize quadratic formula optimization of Renshen Jianxin Capsule（RJC） by screening Chinese herbs with major anti-myocardial ischemia effect in RJC and optimize their optimal dosages.Methods By following ＂uniform design-pharmacodynamic experiment-mathematical modeling-formula optimization＂,authors employed U_（10）（10~8） uniform design in the experiment.Eight Chinese herbs contained in RJC were taken as observatory factors.Electrocardiograph（ECG） changes of myocardial ischemia induced by isoproterenol were taken as pharmacodynamic indices.The mathematical model between herbal factors and pharmacodynamic indices was established using stepwise regression analysis to screen Chinese herbs with major anti-myocardial ischemia effect.Their optimal dosages were optimized using the grid algorithm.Results The regression equation was y =1.7889-0.3247 Ginseng ×Saivia Miltiorrhiza-0.0663 Astragalus membranaceus ×Oriental Waterplantain tuber.Forecasting factors included were Ginseng,Salvia Miltiorrhiza,Astragalus membranaceus,and Oriental Waterplantain tuber.The optimal formula dosage calculated by the grid algorithm was Ginseng 1.62 g,Astragalus membranaceus 4.62 g,Salvia Miltiorrhiza 2.43 g,and Oriental Waterplantain tuber 1.66 g.Conclusion Uniform design combined with stepwise regression analysis and grid algorithm were able to realize quadratic formula optimization of RJC.