皮肤染毒火箭煤油对小鼠外周血象及细胞因子的影响

Effects on blood cell numbers and cytokines of dermal application rocket kerosene in mice

目的 观察小鼠皮肤染毒火箭煤油对小鼠血象和血清中细胞因子的影响.方法 ICR小鼠背部剃毛,随机分为正常对照组和火箭煤油组,分别涂抹麻油和火箭煤油原油（400 μl＆#215;1次）.染毒后2、6、12h和1、3、7d测定外周血中白细胞、红细胞、血红蛋白、血小板、淋巴细胞、单核细胞和中性粒细胞数量和染毒后2、6、12h和1、3、5、7d用流式细胞术的微阵列技术检测血清中白细胞介素（IL）-2、IL-4、IL-6、IL-10、肿瘤坏死因子-α（TNF-α）、干扰素（IFN）-γ和IL-17A的含量.结果 与正常对照组比较,火箭煤油染毒组染毒后6、12h和1d白细胞和淋巴细胞数量明显下降,染毒后7d白细胞和淋巴细胞数量明显增多,差异均有统计学意义（P＜0.05）.与正常对照组比较,火箭煤油组染毒后2、6、12h和1、3 d IL-6含量明显增高,染毒后2h和3、5、7 d IL-10的含量明显增高,差异均有统计学意义（P＜0.05）;火箭煤油组染毒后2、6h和3、5、7 d TNF-α的含量明显增高,染毒后6h和1、3 d IFN-γ的含量明显增高,染毒后3、5、7 d IL-17A的含量明显增高,差异均有统计学意义（P＜0.05）.结论 皮肤染毒火箭煤油可以改变炎症细胞数量,引起机体细胞因子变化.

Objective To detect the number of cells and the level of IL-2,IL-4,IL-6,IL-10,TNF-alpha,IFN-γ and IL-17 cytokines in the peripheral blood of mice exposed to rocket kerosene by skin.Method [CR mice were randomly divided into the normal control group and RK experimental group （400 μl＆#215;1 group）.RK undiluted fuel were applied directly to the dorsal skin of the mice.In control groups were treated with sesame oil （SO）.the number of blood cells were detected by automatic blood cell counter and the level of IL-2,IL-4,IL-6,IL-10,TNF-alpha,IFN-γ and IL-17 cytokines in serum were detected by using flow cytometry and BD CBA Flex set kit.Result Compared with the normal group,WBC and LYM had a decreasing tendency 2 h and decreased significantly 6 h,12 h and 1 d after RK exposure （P〈0.05）.They increased significantly 7 d after RK exposure （P〈0.05）.Compared with the normal group,the level of IL-6 increased significantly 2 h,6 h,12 h,1 d and 3 d （P〈0.05）.The level of TNF-α increased significantly 2h,3d,5d and 7d （P〈0.05）.The level of IL-10 increased significantly 2 h,6 h,3 d,5 d and 7 d （P〈0.05）.The level of IFN-γ increased significantly 6 h and 3 d （P〈 0.05）.The level of IL-17 significantly increased 3 d,5 d and 7d （P〈0.05）.Conclusion RK can change the number of immune cells,causing the immune cytokine changes in mice after RK cutaneous exposure.