冰片对兔角膜上皮细胞的损伤作用

Damage Effect of Borneol on Rabbit Corneal Epithelial Cells

【目的】观察冰片对兔角膜上皮细胞的损伤作用。【方法】以浓度分别为100、200、400μg/m L的冰片作用于兔角膜上皮细胞,采用四甲基偶氮唑盐(MTT)法检测细胞活性,Annexin V-异硫氰酸荧光素/碘化丙啶(Annexin V-FITC/PI)双染法流式细胞仪检测细胞凋亡率;Real-time PCR法检测细胞凋亡相关基因Caspase-3 m RNA的表达。【结果】不同浓度的冰片均可抑制兔角膜上皮细胞的活性;与正常对照组比较,冰片组可增加兔角膜上皮细胞的凋亡率,增强Caspase-3 m RNA的表达(P<0.05或P<0.01)。【结论】冰片能抑制兔角膜上皮细胞的生长,诱导细胞发生凋亡,其机制与增强凋亡相关基因Caspase-3m RNA的表达有关。

Objective To observe the damage effect of borneol on rabbit corneal epithelial cells. Methods After the treatment with borneol at 100, 200, 400 μg·mL^-1 respectively, the viability of rabbit corneal epithelial cells was determined by methyl thiazolyl tetrazolium （ MTT） assay, cell apoptosis was determined by flow cytometry with Annexin V- fluorescein isothiocyanate/propidium iodide （ FITC/PI） staining, and Caspase-3 mRNA expression was detected with real-time reverse transcription polymerase chain reaction （ RT-PCR） . Results Borneol at the concentrations of 100, 200, 400 μg·mL-1 inhibited the activity of rabbit corneal epithelial cells. Compared with the normal control group, borneol increased the rate of apoptosis, and enhanced the Caspase-3 mRNA expression in rabbit corneal epithelial cells （ P〈0.05 or P〈0.01） . Conclusion Borneol can inhibit the proliferation of rabbit corneal epithelial cells, induce cell apoptosis through enhancing the expression of apoptosis-related gene Caspase-3 mRNA.