聚脱氧腺苷酸/还原石墨烯纳米复合膜电化学传感器检测核黄素

Electrochemical Sensor for the Detection of Riboflavin Based on Nanocomposite Film of Polydeoxyadenylic Acid/Reduced Graphene Oxide

以含32个碱基的聚脱氧腺苷酸（A32）作为黏合剂,将水合肼还原的石墨烯（RGO）固定在金（Au）电极表面,制备A32/RGO纳米复合膜电化学传感器.利用循环伏安法和交流阻抗法对传感器制备过程进行表征,并用循环伏安法和微分脉冲伏安法研究了该传感器的电化学行为.研究结果表明,与裸Au电极和A32修饰的Au电极相比,RGO/A32/Au电极对核黄素有较高的电化学活性,其还原峰电流与核黄素的浓度在0.025~2.75μmol/L范围内呈线性关系,检出限（S/N=3）为15 nmol/L.该传感器具有灵敏度高、抗干扰能力强及稳定性好等优点,可用于人尿中核黄素的分析测定.

A novel electrochemical sensor to detect riboflavin was constructed using 32-mer polydeoxyadenylic acid（ A32） as an effective adhesive for binding with the hydrazine hydrate-reduced graphene oxide（ RGO） and the gold（ Au） electrode. The fabrication process of the sensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy,and its electrochemical behaviour was studied by cyclic voltammetry and differential pulse voltammetry. The experimental results showed that compared with the bare Au electrode and the A32/ Au electrode,the RGO / A32/ Au electrode displayed excellent electrochemical activity towards riboflavin,and the cathodic peak current increased linearly with the increasing concentration of riboflavin from0. 025 μmol / L to 2. 75 μmol / L with a low detection limit of 15 nmol / L（ S / N = 3）. The sensor possesses high sensitivity,strong anti-interference ability and good stability,and can be used for the analysis of riboflavin in human urine samples.