菊花不同外植体组培快繁及其再生体系的研究

Research of Tissue Culture and Regenerated of the Different Explants of Chrysanthemum indicum L.

以菊花不同外植体为试材,采用离体培养快速繁殖的方法,研究菊花不同外植体在不同的培养基中诱导不定芽再生的频率。结果表明:菊花不同基因型外植体在含适宜6-BA、NAA等激素的培养基中形成愈伤组织的难易程度不同,愈伤组织诱导形成不定芽的能力强弱也不一样;不同基因型菊花外植体的不定芽诱导率高低排序是花蕾>花瓣>茎段>叶片;对菊花的花蕾、花瓣、茎段和叶片来说最适合的培养基配方是MS+6-BA 2.0mg/L+NAA 0.2mg/L,不同培养基诱导不定芽的能力为花蕾>花瓣>茎段>叶片,其中,菊花花蕾和花瓣的再生能力较强。最合适的生根培养基是1/2MS+NAA 0.2mg/L。该研究成功的建立了菊花不同外植体离体培养再生途径,并获得了再生植株。

Chrysanthemum indicumL.was used as explants to study rapid propagation through tissue culture.The effect of media components on the induction of shoot regeneration for different types of explants in five genotypes of Chrysanthemum indicumL.were investigated.The results showed that forming the callus for different genotypes explants cultured on media containing suitable 1-naphthaleneacetic acid（NAA）and 6-benzylaminopurine（6-BA）concentrations was easy,but shoot regeneration from the callus proved difficult.The frequency of shoot regeneration from chrysanthemum＇s different types of explants for all the genotypes were in turn as follows：flour bud〉petal〉stem〉leaf.The best culture medium for the chrysanthemum＇s explants was MS＋6-BA 2.0mg/L＋NAA 0.2mg/L.The ability of inducing shoot regeneration for different explants of chrysanthemum was very different,and the regenerated frequency of flour bud and petal was the highest.Shooting culture was on 1/2MS medium supplemented with NAA 0.2mg/L.These results indicate that the experiment successfully established the plant regeneration system of Chrysanthemum indicumL.,and successfully obtained the regenerated plant.