栽培料发酵条件及环境条件对瓶栽巨大口蘑菌丝生长影响

Influence of Fermentation and Environmental Conditions on Mycelial Growth of Tricholoma giganteum

以巨大口蘑为试材,通过单因素试验研究栽培料的发酵温度、初始pH值和水分含量对瓶栽巨大口蘑菌丝生长的影响,并采用均匀设计法优化菌丝培养环境条件中的温度、空气相对湿度、二氧化碳浓度,明确巨大口蘑瓶栽所需的必要条件,为实现巨大口蘑从传统的袋子覆土栽培向瓶子无土栽培的转化提供参考依据。结果表明：栽培料的最适发酵温度范围为70-75℃,最适初始pH值为8.5,最适水分含量为61%;从均匀设计法的优化结果得出其菌丝生长的最优环境条件：最适培养温度为30℃,空气相对湿度为60%,二氧化碳浓度为0.15%。

Tricholoma giganteum was taken as test material,the influence of fermentation temperature,initial pH value and water content of the cultivation material on mycelial growth of Tricholoma giganteum was researched by the single factor experiment.The uniform design method was used to optimize the temperature,the relative humidity of the air and the concentration of carbon dioxide of mycelium culture conditions.The objective was to clear the necessary conditions of bottle cultivation and realize Tricholomagiganteum cultivation from the traditional bag cultivation with soil to bottle cultivation without soil.The results showed that from the single factor experiment,the optimal fermentation temperature was 70-75℃,the optimum initial pH value was 8.5,the optimum moisture content was 61%.The optimal environment conditions of mycelial growth from the result of the uniform design experiment was that the optimal temperature 30℃,the optimal relative humidity of the air 60%,and the optimal concentration of carbon dioxide 0.15%.