当归挥发油对吗啡依赖大鼠戒断体征及去甲肾上腺素释放的影响

Effects of angelica naphtha on the withdrawal signs and norepinephrine release in morphine-dependent rats

目的 观察当归挥发油对大鼠吗啡戒断体征及去甲肾上腺素神经递质释放的影响.方法 72只SD大鼠随机均分为空白组、模型组、可乐定组和当归挥发油高（80 mg/kg）、中（40 mg/kg）、低（20 mg/kg）剂量组.采用剂量递增法建立大鼠吗啡依赖模型,采用柳田知司评分法对大鼠戒断体征进行评分;采用酶联免疫吸附法榆测蓝斑核和前额叶皮层中去甲肾上腺素（NE）及其代谢产物间羟去甲肾上腺素（NMN）的含量.结果 空白组、模型组、可乐定组和当归挥发油低、中、高剂量组戒断体征总评分值分别为（4.00±3.29）分、（30.13±4.41）分、（18.96±4.43）分、（26.04±4.13）分、（22.33±4.60）分和（19.00±3.47）分,当归挥发油呈剂量依赖地降低戒断体征总评分值（P＜0.01）,同时显著性降低蓝斑核和前额叶皮层中NE及NMN的含量,降低NMN/NE值（P值均小于0.01）.结论 当归挥发油能够有效缓解大鼠吗啡戒断体征,其机制可能与抑制蓝斑核和前额叶皮层NE周转有关.

Objective To explore the effects of angelica naphtha on the withdrawal signs and norepinephrine neurotransmitter in uorphine-dependent rats.Methods 72 SD rats were randomly divided into control group,model group,clonidine group and three angelica naphtha groups （80,40 and 20 mg/kg）.Rats were received gradualy increasing doses of morphine to produce physical dependence.Withdrawal symptoms were evaluated by Ryuta Tomoji score.The level of norepinephrine and normetanephrine（NMN） were tested by enzyme-linked immunosorbent assay（ELISA）.Results The scores of withdrawal signs were （4.00± 3.29）,（30.13±4.41）,（18.96± 4.43）,26.04±4.13）,（22.33±4.60） and （19.00±3.47）,respectively.The morphine withdrawal signs were reduced by angelica naphtha in a dose-dependent manner（P〈0.01）,also significantly reduced the level of NE and NMN and the ratio of NMN/NE in the nucleus ceruleus and prefrontal cortex（all P〈0.01）.Conclusion Angelica naphtha alleviates the withdrawal synlptons in morphine-dependent rats,which may be related to the inhibition of excessive turnover of norepinephrine neurotransmitters in the nucleus ceruleus and prefrontal cortex.