长链非编码RNA-HOTAIR对前列腺癌细胞周期和侵袭影响的实验研究

Effects of long non-coding RNA-HOTAIR on the cell cycle and invasiveness of prostate cancer

目的:探讨长链非编码RNA(lncRNA)——Hox基因的反义基因间RNA(HOTAIR)在前列腺癌细胞的表达情况并明确其对前列腺癌细胞生长及转移的影响。方法:采用定量RT-PCR(qRT-PCR)检测HOTAIR在人正常前列腺上皮细胞株RWPE-1和前列腺癌细胞株PC3和DU145中的表达水平;采用RNA干扰技术、流式细胞术和Transwell小室实验检测HOTAIR对前列腺癌细胞生长周期及侵袭能力的影响。结果:与人正常前列腺上皮细胞相比,前列腺癌细胞株PC3和DU145中HOTAIR表达分别增高3.2及5.7倍;在si-HOTAIR下调HOTAIR的表达水平后,前列腺癌细胞株均出现了G2期阻滞,同时伴有G1期下调;且前列腺癌细胞株的侵袭能力受到了明显的抑制,si-HOTAIR1和si-HOTAIR2处理后,PC3侵袭能力分别被抑制32%和44%,DU145被抑制43%和34%。结论:lncRNA-HOTAIR在前列腺癌中呈相对高表达,并且与前列腺癌细胞的生长及侵袭能力有关。HOTAIR有潜力成为诊断前列腺癌和判断其预后的新指标。

Objective ： To investigate the expression of long non-coding RNA-HOTAIR in prostate cancer cells and its effects on the growth and metastasis of the cells. Methods： Using quantitative reverse-transcription PCR （qRT-PCR）, we determined the rela- tive expression of HOTAIR in the normal human prostate epithelial cell line RWPE-1 and prostate cancer cell lines PC-3 and DU145. We detected the effects of HOTAIR on the cell cycle and invasiveness of prostate cancer cells by RNA interference, flow cytometry, and Transwell mitration assay. Results ： The expressions of HOTAIR in the PC3 and DU145 ceils were increased 3.2 and 5.7 times, respectively, as compared with that in the normal RWPE-1 cells. After si-HOTAIR interference, the prostate cancer cells were arrested in the （;2 phase and downregulated in the G1 phase. The invasive ability of the prostate cancer cells was evidently inhibited, with the inhibition rates of 32% and 44% of the PC3 cells and 43% and 34% of the DU145 cells for si-HOTAIR1 and si-HOTAIR2, respec- tively. Conclusion ： lncRNA HOTAIR is highly expressed in prostate cancer, which is associated with the growth and invasiveness of prostate cancer cells. HOTAIR is potentially a novel marker for the diagnosis and prognosis of prostate cancer.