糖尿病胃轻瘫大鼠模型制作的实验研究

Experimental Study on the Model of Diabetic Gastroparesis Rat

目的:探讨小剂量链脲佐菌素注射液(STZ)联合高能量饮食法诱导糖尿病胃轻瘫(DGP)大鼠模型。方法:将24只大鼠随机均分为正常组和模型组,正常组大鼠予普通饲料喂养,模型组大鼠予高脂高糖喂养,模型组大鼠高脂高糖喂养4周后,予1%STZ溶液腹腔注射3次,剂量分别为20、25、35 mg/kg,第3次注射后4周测大鼠空腹血糖、胃内残留率、血清胃动素(MLT)、胃泌素(GAS),同时取大鼠胃组织,观察胃窦平滑肌细胞。结果:模型组大鼠空腹血糖、胃内残留率、MLT、GAS明显高于正常组,差异具有统计学意义(P<0.01);模型组大鼠胃窦平滑肌细胞形态学改变较正常组大鼠有明显差异。结论:小剂量STZ联合高能量饮食法可成功复制DGP大鼠模型。

Objective： To investigate the establishment of rat model of diabetic gastroparesis （DGP） by low dose of STZ combined with high energy diet. Methods： Twenty-four rats were randomly divided into normal group and model group, 12 rats in each group. Rats in normal group were fed with common diet while rats in model group were fed with high energy diet. After four weeks of high energy diet feeding, rats in model group received intraperitoneal injection of low dose of STZ solution three times, 20, 25, 35 mg/kg respectively. Four weeks after the third injection, FBG level, the gastric emptying rate, serum MLT, GAS content of rats were detected. And the gastric tissue of rats was taken out to observe nlorphological changes of gastric smooth muscle cell. Results： The FBG level, gastric empty- ing rate, serum MLT and GAS content of rats in model group were significantly higher than their coun- terparts in normal group （P 〈 0.01 ）. The morphological changes of gastric smooth muscle cell in mod- el group were different from those in normal group. Conclusion： Low dose of STZ combined with high energy diet can successfully establish the rat model of DGP.