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成牙骨质细胞在缺氧条件下破骨功能调节的相关研究

Effect of Hypoxia to Osteoclast Function of Cementoblast-like Cell
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摘要 目的探讨成牙骨质样细胞OCCM-30在不同时间缺氧微环境下破骨功能的调节变化。方法利用三气培养箱构建细胞缺氧模型,以常氧条件培养为对照组,应用RT-PCR检测缺氧后OCCM-30细胞骨桥蛋白(OPN)、骨保护素(OPG)、核因子κB受体活化因子配体(RANKL)mRNA表达的变化。结果 OPN mRNA的表达随缺氧时间逐步上调,36h达到顶峰后进入平台期。OPG mRNA的表达,随着缺氧的开始快速上调,在12h达到顶峰后进入平台期,在36h后逐步下降;RANKL mRNA的诱导表达呈现先上升后下降的趋势。结论缺氧微环境能显著诱导OCCM-30细胞表达破骨调节功能。 Objective To investigate the influence of hypoxia to the osteoclast function related gene of OCCM-30 cell. Methods Use special incubator to construct hypoxic model, take the cell cultured under normal oxygenic pressure as control and use RT-PCR method to measure the genetic expression of OPN, OPG and RANKL mRNA. Results The expression of OPN mR-NA could be induced by hypoxia and reached the highest point at 36h;the expression of OPG mRNA could be induced quickly by hypoxia and reached the highest platform at 12h, then decreased after 36h;the expression of RANKL mRNA showed a downward trend after the first rise. Conclusion The hypoxia could obviously induced osteoclast function of OCCM-30 cell.
作者 吴浩 韩红娟 任小华 梁琳
机构地区 四川省医学科学院.四川省人民医院口腔科药学部
出处 《四川医学》 CAS 2015年第9期1253-1255,共3页 Sichuan Medical Journal
关键词 缺氧 成牙骨质样细胞 破骨功能 hypoxia cementoblast-like cell osteoclast function
分类号 R783.5 [医药卫生—口腔医学]
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参考文献15

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四川医学
2015年 第9期

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