马铃薯淀粉合成关键酶与块茎淀粉积累的关系

Relationship Between Key Enzymes of Starch Synthesis and Tuber Starch Accumulation

为探讨淀粉在马铃薯块茎中积累规律,及催化其生物合成的4种关键酶。从而为马铃薯的淀粉品质改善提供合理依据。以淀粉含量不同的马铃薯品种‘东农308’和‘延薯4号’为材料,在马铃薯匍匐茎顶端膨大后开始测定马铃薯叶片与块茎中的淀粉合成关键酶、淀粉动态积累量以及直链淀粉动态积累量。研究结果表明,这4种关键酶在淀粉合成积累过程的不同时期表现出不同的活性强弱,继而控制淀粉生物合成的开始与速率,并影响马铃薯直链、支链淀粉的合成与积累。且‘东农308’淀粉含量以及淀粉各组分含量在各时期都要高于‘延薯4号’,这也是由于‘东农308’的淀粉合成关键酶活性高于‘延薯4号’的酶活性。

The experiment used two potato varieties‘Dongnong 308’and‘Yanshu 4’as tested materials, andaimed to research the absorption and accumulation of starch in tubers of different potato varieties, and to studythe four key enzymes related to its synthesis and provide a reasonable basis for potato improvement. After thetop of the potato stolonic began to expand, the key enzymes of starch synthesis, the dynamic accumulation ofstarch and the amylose dynamic accumulation in leaf blade and tubers were measured. The results showed that,the four key enzymes in different periods of starch accumulation and synthesis process showed different activitystrength, which in turn controlled the beginning and the rate of the starch biosynthesis, and then influenced thesynthesis and accumulation of potato amylose and amylopectin. And both the starch content and starch contentof each component of‘Dongnong 308’were more than that of‘Yanshu 4’in every period. The reason was thatthe key enzymes of starch synthesis of‘Dongnong 308’were higher than that of‘Yanshu 4’.