环氧合酶-1和环氧合酶-2基因多态性与阿司匹林抵抗的相关性研究

Correlation between cyclooxygenase-1,2 gene polymorphism and aspirin resistance

目的探讨环氧合酶-1(COX-1)、环氧合酶-2(COX-2)基因多态性与阿司匹林抵抗相关性。方法将96例缺血性脑卒中患者分为阿司匹林敏感组87例和阿司匹林非敏感组9例。2组患者口服100 mg阿司匹林至少一周。用光比浊法对患者的血小板聚集度进行测定,用Sequenom Mass A RRAY i PLEX基因型分析技术进行COX-1、COX-2的基因型检测,分析COX-1、COX-2基因多态性与阿司匹林的相关性。结果阿司匹林非敏感组患者的既往卒中史比例66.7%(6/9)显著高于阿司匹林敏感组21.9%(19/87)(P<0.05)。2组患者的COX-1(rs1330344,rs3842788)基因型分布差异有统计学意义(P<0.05),但COX-1(rs5788)、COX-2(rs20417)基因型分布差异无统计学意义(P>0.05)。COX-1(rs5788,rs1330344,rs3842788)、COX-2(rs20417)等位基因及基因型频率4个单核苷酸基因多态性均符合Hardy-Weinberg平衡(P>0.05)。结论 COX-1(rs3842788,rs1330344)GG型与阿司匹林抵抗密切相关。

Objective To investigate the correlation between cyclooxygenase- 1（ COX- 1）,cyclooxygenase- 2（ COX- 2） gene polymorphism and aspirin resistance. Methods Ninety six patients with ischemic stroke were divided into aspirin- sensitive group（ n = 87） and aspirin-insensitive group（ n = 9）. The patients in two groups were given 100 mg aspirin by oral administration forat least a week. Platelet aggregation was measured by light nephelometry genotypings of COX- 1,COX- 2 were detected by Sequenom Mass A RRAY i PLEX genotyping technology and the correlation of gene polymorphism and aspirin were analyzed. Results The previous history of stroke ratio of aspirin- insensitive group was66. 7%（ 6 /9）,significantly higher than that of aspirin- sensitive group（ 21. 9%,19 /87）（ P〈 0. 05） The COX- 1 genotypes（ rs1330344,rs3842788） between two groups were significantly different（ P 〉0. 05）,but the difference of COX- 1（ rs5788）,COX- 2（ rs20417） was not significant（ P〉 0. 05）. COX- 1（ rs5788,rs1330344,rs3842788）,COX- 2（ rs20417）,etc. allele and genotype frequencies of 4 SNPs were consistent with Hardy- Weinberg equilibrium（ P 0. 05）.Conclusion COX- 1（ rs3842788,rs1330344） GG type is closely related to aspirin resistance.