α-细辛醚对大鼠海马星型胶质细胞活化的影响

Effects of α-asarone on activation of astrocyte of hippocampus in rat

目的研究α-细辛醚对大鼠星型胶质细胞活化的影响。方法诱发大鼠癫痫持续状态(SE)1 h,用胶质纤维酸性蛋白(GFAP)免疫组化染色观察正常组、模型组和实验组的大鼠海马区的星型胶质细胞活化情况;原代大鼠海马星型胶质细胞传代后,分为正常组、脂多糖诱导活化模型组和实验组;用CCK-8试剂盒检测各组细胞的增殖情况,用Western-blotting法检测各组GFAP表达。结果模型组在SE后第1天的海马星型胶质细胞活化最明显,在第28天恢复至正常水平;实验组的星型胶质细胞活化水平在第1天、第3天和第7天均低于模型组(P<0.05)。高、低2个剂量(50,100μg·m L-1)α-细辛醚对体外脂多糖诱导的星型胶质细胞的增殖有明显抑制作用(P<0.05);高浓度实验组对脂多糖诱导星型胶质细胞的GFAP表达增多有明显抑制作用(P<0.05)。结论α-细辛醚对大鼠海马星型胶质细胞的活化有抑制作用。

Objective To investigate the effects of α- asarone on activation of astrocytes of rat hippocampus. Methods Rat status epilepticus（ SE） lasted for 1 hour. The activations of astrocyte in rat hippocampus of control group,model group and α- asarone treatment group were observed by Glial fibrillary acidic protein（ GFAP） immunohistochemistry.The astrocytes of rat hippocampus after passage were randomly assigned to control group,lipopolysaccharide（ LPS） model group and α- asarone treatment group. Then the ability of cell proliferation were detected by CCK8. The GFAP were detected by Western- blotting. Results The most obvious activation of astrocyte in hippocampus of post- SE rats was observed at 1 d,then back to normal at 28 d after SE. Compared with model group,the activated levels of astrocyte in α- asarone treatment group were significantly decreased at 1,3,7 days after SE（ P 0. 05）.Compared with model group,proliferation of astrocyte induced by LPS were significantly decreased in 50 μg·m L- 1and 100 μg·m L- 1of α-asarone treatment groups and GFAP expression were significantly decreased in 100 μg·m L- 1α- asarone group（ P〈 0. 05）. Conclusionα- Asarone could decreased the activation of astrocyte of rat hippocampus.