HPLC法同时测定枣叶中5种黄酮的含量

Determination of five flavonoids in jujube leaves by HPLC

建立了一种枣叶中黄酮类成分定量分析的HPLC方法,并对14个品种的大枣及酸枣叶中这5种黄酮的含量进行了分析。结果得到采用Hypersil BDS C18色谱柱,以乙腈与0.1%甲酸水溶液为流动相梯度洗脱,流速1 m L/min,柱温30℃,检测波长360 nm,实现了槲皮素-3-O-洋槐糖苷、芦丁、槲皮素-3-O-β-D-葡萄糖苷、山奈酚-3-O-芸香糖苷、槲皮素-3-O-α-L-阿拉伯糖-（1→2）-α-L-鼠李糖苷5种黄酮苷的同时检测,最低检出限为1.44~2.59μg/m L,加标回收率在93.8%~105.9%之间。5种黄酮苷的含量测定结果表明不同品种的枣叶中黄酮类成分的组成和含量差异较大,其中芦丁和槲皮素-3-O-洋槐糖苷的含量较高,平均为7.83 mg/g D.W和4.61 mg/g D.W;8个品种枣叶中检测到槲皮素-3-O-α-L-阿拉伯糖-（1→2）-α-L-鼠李糖苷,平均5.32 mg/g D.W;而槲皮素-3-O-β-D-葡萄糖苷和山奈酚-3-O-芸香糖苷的含量较低,分别为0.14 mg/g D.W和0.41 mg/g D.W。

The purpose was to establish a method for determining flavonoids in jujube leaves using HPLC,and analyze flavonoids contents in jujube leaves from 14 different cultivars by this method. The samples were separated on a Hypersil BDS C18 column,the mobile phase consisted acetonitrile and water with 0.1% formic acid, separations were achieved by a gradient elution program, the flow rate was 1 m L / min, the column temperature was 30 ℃ and the detection wavelength was at 360 nm. Under these conditions,five flavonoids including quercetin-3-O-robinobioside,rutin,quercetin-3-O-β-D-glucoside,kaempferol-3-O-rutinoside and quercetin-3-O-α-L-arabinosyl-（1→2）-α-L-rhamnoside were detected by one injection. The minimum detection limit was 1.44~2.59 μg/m L. The average recovery rate was 93.8%~105.8%. The results revealed that in terms of flavonoids,the difference of the composition and contents among the different cultivars was siginificant. The average contents of rutin and quercetin-3-O-robinobioside were 7.83 and 4.61 mg/g D.W,respectively,which were higher compared with other flavonoids. Only 8 cultivars detected the existence of quercetin-3-O-α-L-arabinosyl-（1 →2）-α-L-rhamnoside,whose average contents was 5.32 mg/g D.W.However,the contents of quercetin-3-O-β-D-glucoside and kaempferol-3-O-rutinoside were lower,they were 0.14 and 0.41 mg/g D.W,respectively.