响应曲面法优化茶叶籽油超声辅助LVK脂肪酶酶解工艺

Response surface optimization of ultrasonic-assisted tea seed oil hydrolysis catalyzed by lipase LVK

对茶叶籽油的超声波辅助LVK脂肪酶酶解工艺进行优化。在单因素实验基础上,选取LVK脂肪酶浓度、初始p H、酶解温度和时间为考察因子,茶叶籽油水解率为响应值,运用中心组合实验设计对其酶解工艺进行优化,并建立数学回归模型。结果表明,优化工艺条件为:超声功率220 W,搅拌转速800 r/min,油水比1∶1.75(w/v),氯化钙浓度0.25%,LVK脂肪酶浓度5.5%,酶解温度49.5℃,初始p H9.4,酶解时间4 h。在此条件下,水解率实测值为83.15%,模型预测值为82.06%,水解效率高。

In order to optimize the method of tea seed oil enzymatic hydrolysis by ultrasonic-assisted. Based on the single factor experiments,LVK lipase dosage,initial p H value and reaction temperature ＆ time were selected as trial factors,the hydrolysis rate of tea seed oil was the response value,a central composite design was used to obtain a mathematical regression model for optimize the parameters of tea seed oil hydrolysis by lipase under ultrasonic. The results showed that the optimal parameters were obtained as follows：ultrasonic power 220 W,stirring speed 800 r/min,oil to water ratio 1∶1.75（w/v）,Ca Cl2 dosage 0.25%,LVK lipase dosage5.5%,temperature 49.5 ℃,initial p H value 9.4,reaction time 4 h. Under these conditions,the experimental hydrolysis rate was 83.15% and the model value predicted was 82.06% and a high efficiency of hydrolysis was exhibited in this study.