复方功能制剂对改善小鼠记忆功能的影响

Effect of the compound preparation on improving the memory of mice

为研究牛磺酸、咖啡因、人参皂甙复方功能制剂在改善记忆方面的功能,选取雄性昆明小鼠40只,随机分为空白对照组、低剂量组、中剂量组和高剂量组。低、中、高剂量组每天分别灌胃给予复方功能制剂41、80和237 mg/kg BW,空白对照组每天灌胃给予同体积的蒸馏水,共灌胃30 d。末次灌胃后次日将4组小鼠按跳台实验、避暗实验、穿梭箱实验、Morris水迷宫实验顺次进行实验,比较组间各项观察指标的差异。研究结果表明,中、高剂量组跳台实验记忆巩固潜伏期显著延长(p<0.05),低、中、高剂量组穿梭实验不同训练周期的主动/被动回避时间、水迷宫实验的对位探查实验均有显著性差异(p<0.05)。提示本复方功能制剂能显著改善小鼠的学习记忆能力,具有改善记忆功能。

This study was designed to evaluate effects of taurine,caffeine and ginsenosides compound preparation on improving the memory of mice. Forty Kunming male mice were randomly divided into four groups,including groups of low-dosage,medium-dosage,high-dosage and control（each with n=10）. The low,medium and high dosage groups were given 41,80 and 237 mg/kg BW of compound preparation respectively through intragastric intubation daily for 30 days. The control group was given the same volume of distilled water at the same time. Experiments of step-down test,step through test,shuttle box test and Morris water maze test were conducted in order after the last intubation. The differences between each group were compared. Results indicated that the memory consolidation latent period of medium and high dosage groups were prolonged significantly in step down test（p〈0.05）. In shuttle box test,the active and passive evading time of the low,median and high dosage groups in all training cycles shortened（p〈0.05）. In water maze test,the counterpoint exploring time also were shortened（p〈0.05）. In conclusion,the compound preparation could improve significantly the learning and memory ability of mice. The results indicated that the compound preparation had memory improving effect.