长链非编码核糖核酸UCA-1对胰腺癌细胞侵袭转移能力的影响

Effects of long non-coding RNA UCA-1 on the capacity of invasion and metastasis of pancreatic cancer cells

目的探讨尿路上皮癌胚抗原1(UCA-1)在胰腺癌细胞侵袭转移中的作用。方法采用实时荧光定量PCR检测5株胰腺癌细胞长链非编码核糖核酸UCA-1的表达;采用UCA-1表达质粒提高PaTu8988细胞的UCA-1水平,小干扰RNA用于降低BxPC-3细胞的UCA-1表达。用Transwell侵袭实验和迁移实验观察转染后的PaTu8988细胞和BxPC-3细胞的侵袭和迁移能力变化,Western blot检测基质金属蛋白酶2(MMP-2)和MMP-9的表达。结果 UCA-1差异性表达于5株胰腺癌细胞。过表达UCA-1后,PaTu8988细胞的MMP-2和MMP-9的蛋白水平均增加,侵袭和迁移能力增强(P<0.001);而干扰UCA-1后,BxPC-3细胞的MMP-2和MMP-9的蛋白表达均降低,侵袭和迁移能力减弱(P<0.001)。结论 UCA-1的高表达能增强胰腺癌细胞的体外侵袭转移能力。

Objective To explore the role of long noncoding RNA urothelial carcinoma antigen 1 （UCA1） in the invasion and metastasis of pancreatic cancer cells. Methods The expressions of UCA1 in 5 pancreatic cancer cell lines were analyzed by qRT PCR, The UCA1 plasmid was used to overexpress the UCA-1 in PaTu8988 cells. The level of UCA-1 in BxPC-3 cells was knocked down by small interfering RNA（siRNA）. The capacity of invasion and migration in vitro of transfeeted PaTu8988 and BxPC-3 cells was tested by Transwell invasion assay and migration assay. The protein levels of matrix metalloproteinase 2（MMP-2） and MMP-9 were examined by Western blot. Results UCA-1 was differentially expressed in 5 pancreatic cancer cell lines. Upregulation of UCA-1 increased the levels of MMP-2 and MMP-9 and enhanced the ability of invasion and migration of PaTu8988 cells. Downregulation of UCA-1 decreased the expressions of MMP-2 and MMP-9 and impaired the capacity of invasion and migration of BxPC-3 cells（P^0. 001）. Conclusion The overexpression of UCA-1 can enhance the ability of invasion and metastasis in vitro of pancreatic cancer ceils.