肝细胞肝癌中神经上皮细胞转化基因1及皮质肌动蛋白表达及其意义

Expression and significance of NET-1 and cortactin in hepatocellular carcinoma

目的探讨肝细胞肝癌(HCC)神经上皮细胞转化基因1(NET-1)及皮质肌动蛋白表达水平与HCC患者预后的关系。方法用免疫组化SP法检测103例石蜡标本HCC组织及57例癌旁组织中NET-1和皮质肌动蛋白表达,分析其与临床病理特征和生存率之间的关系。结果 HCC组织中,NET-1和皮质肌动蛋白阳性表达率分别为85.96%和73.68%,高于癌旁组织的59.60%和28.10%(P<0.01)。HCC组织NET-1表达与肿瘤大小、分级、TNM分期和肝硬化背景有关(P<0.05或P<0.01)。皮质肌动蛋白表达与肿瘤包膜完整性、脉管转移、TNM分期有关(P<0.05或P<0.01)。HCC中,NET-1和皮质肌动蛋白表达呈显著正相关(r=0.280,P<0.05)。NET-1和皮质肌动蛋白表达均阳性组5年生存率低于两者均阴性组(27.78%vs.54.54%)(P<0.05)。结论 HCC中的NET-1和皮质肌动蛋白表达增加,可作为评价HCC肿瘤分级、分期及判断预后的重要参考指标。

Objective To investigate the expression of neuroepithelial cells transforming gene-1 （NET-l） and cortactin and their correlation with clinicopathological features and prognosis in the patients with hepatocellular carcinoma（HCC）. Methods The expressions of NET-1 and cortactin were detected by immunohistochemistry in 103 paraffin specimens of HCC tissues（group A） and 57 matched tumor-adjacent liver tissues（group B）. The relationship of NET-1 and cortactin expressions with clinicopathological features and prognosis was analyzed. Results The expressions of NET-1 and cortactin were higher in group A than those in group B（85.96% vs. 59.60% and 73.68% vs. 28. 10%）（P〈0. 01）. The expression of NET-1 in HCC was closely related with HCC grade, tumor size,TNM stage and cirrhosis background（P〈0. 05 or P〈0. 01）. The expression of cortactin was closely related to tumor membrane integrity, vascular invasion and TNM stage （P〈0.05 or P〈0. 01）. The expression of cortactin was positively correlated to that of NET-1 （r=0. 280, P〈0. 05）. The 5-year survival rate of patients with positive expressions of NET-1 and cortactin was lower than those with negative expressions of NET-1 and cortactin（27.78% vs. 54.54o/oo） （P〈0. 05）. Conclusion NET-1 and cortactin are overexpressed in HCC and may be used as the indicators for the evaluation of TNM stage and prognosis.