HPLC法同时测定加味香连丸中芍药苷、柚皮苷、橙皮苷和黄芩苷

Simultaneous determination of paeoniflorin,naringin,hesperidin and baicalin in Jiawei Xianglian Pills by HPLC

目的建立同时测定加味香连丸中芍药苷、柚皮苷、橙皮苷和黄芩苷的HPLC法。方法采用Hypersil ODS色谱柱（4.6 mm×200 mm,5μm）;乙腈-0.2%磷酸水溶液为流动相;体积流量1.0 m L/min;检测波长274 nm;柱温为25℃。结果加味香连丸中的芍药苷、柚皮苷、橙皮苷和黄芩苷分别在0.32~7.68μg/m L（r=0.999 8）、2.08~49.92μg/m L（r=0.999 8）、0.68~16.32μg/m L（r=0.999 7）和1.32~31.68μg/m L（r=0.999 8）范围内与峰面积呈良好的线性关系;平均回收率分别为98.2%（RSD为0.88%）、97.7%（RSD为1.3%）、98.7%（RSD为0.94%）和97.9%（RSD为0.90%）。结论本方法适用于加味香连丸中4种成分的定量测定。

AIM To establish an HPLC method for simultaneous determination of paeoniflorin,naringin,hesperidin and baicalin in Jiawei Xianglian Pills. METHODS Using Hypersil ODS column（ 4. 6 mm × 200 mm,5μm）,mobile phase was acetonitrile-0. 2% phosphoric acid solution with a flow rate of 1. 0 m L /min,the detection wavelength was 274 nm,and the column temperature was 25 ℃. RESULTS The calibration curves were linear in the ranges of 0. 32- 7. 68 μg / m L（ r = 0. 999 8） for paeoniflorin,2. 08- 49. 92 μg / m L（ r = 0. 999 7） for naringin,0. 68- 16. 32 μg / m L（ r = 0. 999 5） for hespiridin and 1. 32- 31. 68 μg / m L（ r = 0. 999 7） for baicalin,the average recoveries of these chemical constituents were 98. 2%（ RSD = 0. 88%）,97. 7%（ RSD = 1. 3%）,98. 7%（ RSD = 0. 94%） and 97. 9%（ RSD = 0. 90%）,respectively. CONCLUSION This method is applicable for the determination of these four constituents in Jiawei Xianglian Pills.