摘要
目的研究uPA(尿激酶型纤溶酶原激活物)蛋白、mRNA在子宫内膜异位症中的表达,并分析其在该病中发挥的作用。方法采用RT-PCR的试验方法检测u PA蛋白在对照组子宫内膜(来源于同期子宫肌瘤患者)、子宫内膜异位症患者在位、异位子宫内膜各35例中的表达情况,采用酶联免疫吸附实验(ELISA)分别检测子宫内膜异位症患者及对照组患者血清各35例中u PA蛋白的表达。结果 u PA、mRNA在子宫内膜异位症患者异位子宫内膜中的表达高于在位子宫内膜,且其在两者中的表达均高于正常子宫内膜,差异均有统计学意义(P<0.05);u PA蛋白在子宫内膜异位症患者血清中的表达明显高于对照组,差异有统计学意义(P<0.05)。结论 u PA蛋白及mRNA水平在子宫内膜异位症患者子宫内膜中均高表达,其可能参与了子宫内膜异位症病理的形成。
Objective To investigate the expression of uPA（urokinase- typeplasminogen activator） protein,mRNA in endometriosisand and to analyze its role in it. Methods The expression of uPA mRNA was detected by RT- PCR in 35 cases normal endometrium,endometrial eutopic and ectopic endometrium. The expression of uPA protein was detected by ELISA in serum of 35 cases endometrium patients and uterine fibroids in patients. Results The expression of uPA mRNA in patients with ectopic endometriosis,eutopic endometrium were higher than normal endometrium,which the differences were statistically significant（P〈0. 05）. The expression of uPA protein in the serum of patients with endometriosis was significantly higher than patients with uterine fibroids,which the difference was statistically significant（P〈0. 05）. Conclusion uPA is highly expressed in patients with endometriosis and it may be the key point of the important factors leading to the disease and drug targeting.
出处
《宁夏医学杂志》
CAS
2015年第9期769-770,共2页
Ningxia Medical Journal
基金
国家自然科学基金资助项目(81160078)
