利用HPCE构建小麦LMW-GS标准图谱初探

Preliminary Construction of Standard LMW-GS Profile in Common Wheat Using HPCE

为给不同小麦品种低分子量谷蛋白亚基(LMW-GS)的鉴定、品质预测提供快速、准确的方法,以黄淮麦区14个普通小麦品种为材料,首先利用SDS-PAGE和分子标记确定LMW-GS组成,然后采用改进的HPCE体系分离LMW-GS,最后通过控制变量法比对小麦品种间相同亚基以判断各LMW-GS亚基在图谱中对应的峰。结果表明,采用SDS-PAGE和分子标记相结合的方法可以更加准确地鉴定小麦LMW-GS;利用HPCE体系获得的小麦LMW-GS图谱具有很高的重复性,重复试验所得小麦LMW-GS图谱中各个峰迁移时间的相对标准偏差均在0.30%以下;通过控制变量法所建立的小麦LMW-GS图谱可以实现Glu-A3a、GluA3c、Glu-A3d、Glu-B3a、Glu-B3d、Glu-B3h的快速鉴定。

To provide a fast and accurate way for identification of Low-molecular-weight glutenin sub- units （LMW-GS） and quality prediction from different wheat varieties, 14 common wheat varieties from Huang-Huai wheat region were researched in this study. SDS-PAGE and molecular markers were used to identify the compositions of LMW-GS in 14 wheat varieties ; improved HPCE system was used to separate LMW-GS, controlling variable method was used to identify the corresponding peaks of LMW-GS in profiles by comparing same subunits from different varieties. The results showed that the combination of SDS-PAGE and molecular markers can identify LMW-GS accurately l the repeatability of HPCE profile is high and the relative standard deviation（RSD） of migration time of every peak in duplicate test is less than 0. 3 %the developed HPCE profile can identify GlwA 3a, Glu-A 3c, Glu- A 3d,Glu-B 3a,GlwB 3d,GlwB 3h rapidly.