二氯甲烷对小鼠肝功能影响的研究

Study on the effect of dichloromethane on liver function in mice

目的探讨二氯甲烷(DCM)染毒对小鼠血清中肝功能及肝组织病理的影响。方法将昆明种雄性小鼠随机分为高、中、低剂量染毒组(染毒剂量分别为12 900、6 400、1 300mg/m3)和对照组(0mg/m3),每组6只。将小鼠成笼放入动式染毒室内,每天染毒2h,连续染毒30d后,检测血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、总抗氧化能力(T-AOC)水平;取小鼠肝脏作石蜡切片,HE染色观察肝脏组织病理学变化。结果与对照组比较,各染毒组ALT、AST、ALP水平均有升高,差异均有统计学意义(P<0.05);与对照组比较,各染毒组SOD、MDA含量均有升高(P<0.05),GSH含量、T-AOC水平随染毒剂量的增加呈下降趋势(P<0.05);组织病理切片显示小鼠肝脏出现炎症反应。结论 DCM吸入染毒对小鼠的肝脏功能有一定的影响,并出现炎症性病理变化。

Objective To explore the hepatic toxicity of dichloromethane（DCM）on male mice.Methods24 male mice were divided into four groups,control（0mg/m3）,low（1 300mg/m3）, moderate（6 400mg/m3）and high（12 900mg/m3）dose groups in the test.The mice were treated with DCM inhaled2 hper day for 30 consecutive days.The blood serum was collected to observe alanine aminotransferase（ALT）,aspartate transaminase（AST）,alkalinephosp hatase（ALP）,malondialdehyde（MDA）,superoxide dismutase（SOD,glutathione（GSH）content and total antioxidant capacity（T-AOC）activity.Results Compared with the control group,ALT,AST,ALP,MDA,SOD of the male mice in the other three dose groups increased（P〈0.05）,GSH content and T-AOC activity decreased（P〈0.05）.Liver tissue of the male mice in the low,moderate and high dose groups showed obviously abnormal in pathology test.Conclusions The study suggested that DCM could negatively affect the liver function of mice,and the liver of male mice tends to show signs of inflammatory response.