糖皮质激素诱导的肿瘤坏死因子受体及其配体mRNA在哮喘大鼠中性粒细胞上的表达及布地奈德干预的研究

Effect of budesonide treatment on the expression of GITR / GITRL mRNA in polymorphonuclear leukocyte of asthmatic rats

目的:观察布地奈德对哮喘大鼠外周血中性粒细胞上糖皮质激素诱导的肿瘤坏死因子受体(GITR)及其配体(GITRL)表达水平的影响。方法:将30只清洁级SD大鼠随机分为3组:对照组、哮喘组、布地奈德干预组,取外周血进行中性粒细胞的分离提纯,采用实时荧光定量逆转录聚合酶链反应(QRT-PCR)方法检测GITR/GITRL mRNA在中性粒细胞上的表达。结果:哮喘组中性粒细胞上的GITR/GITRL mRNA的表达水平(△CT分别为3.10±0.23,13.48±2.40)显著高于对照组(△CT分别为5.16±0.70,15.79±1.27)及布地奈德干预组(△CT分别为4.83±0.93,15.63±1.73)(均P<0.05);而布地奈德干预组与对照组相比,差异无统计学意义(P>0.05)。对各组的GITR/GITRL mRNA表达水平进行直线相关分析,结果显示两者表达呈正相关(R2=0.669,P<0.01)。结论:中性粒细胞可能通过GITR/GITRL信号通路参与哮喘的气道炎症过程,布地奈德可能通过下调中性粒细胞上的GITR/GITRL的表达从而减轻哮喘气道炎症反应。

AIM： To explore the effect of budesonide on the expression of glucocorticoid-induced tumor necrosis factor recepetor / glucocorticoidinduced tumor necrosis factor ligand（ GITR /GITRL） in polymorphonuclear leukocyte（ PMN）from peripheral blood in asthmatic rats. METHODS： Thirty Clean-level SD rats were randomly divided into three groups including control group,asthma group,budesonide-treatment group. The expressions of GITR / GITRL mRNA in PMN of peripheral blood were detected by real-time PCR in asthrnatic rats. RESULTS： The expressions of GITR /GITRL mRNA（ △CT 3. 10 ± 0. 23,13. 48 ± 2. 40）in asthma group were significantly higher than those of control group（ △CT 5. 16 ± 0. 70,15. 79 ± 1. 27）or budesonide-treatment group（ △CT 4. 83 ± 0. 93,15. 63 ± 1. 73）（ all P〈0. 05）. However,there was no statistically significant difference in the relative expression of GITR / GITRL mRNA between the control group and budesonide-treatment group（ P〈0. 05）. Linear correlation analysis on GITR / GITRL expression level showed positive correlation（ R2=0. 669,P〈0. 01）. CONCLUSION： We demonstrated that probably PMN may participate in the pathogenesis of asthma in rats via GITR / GITRL signals. And budesonide may through inhibiting GITR / GITRL pathway in PMN relieve asthma airway inflammation.