CL_2 MDP脂质体的制备及对小鼠肝脏Kupffer细胞的作用

Preparation of CL_2 MDP liposomes and its function in Kupffer cells from the liver of mice

目的:建立制备氯磷酸二钠(CL2MDP)脂质体的方法,并观察其剔除小鼠肝脏Kupffer细胞的效果。方法:采用脂质体包裹CL2MDP,分光光度计检测包封率;并给BALB/c小鼠尾静脉注射(0.1 m L/10 g);3 d后,免疫组化检测小鼠Kupffer细胞的数量(抗小鼠F4/80表达阳性),流式细胞仪检测分离后肝脏贴壁细胞表达的CD11c,数据统计分析。结果:CL2MDP包封率(%)为20.1±2.9;免疫组化结果,小鼠Kupffer细胞数量在PBS组为(16.2±1.8)个,CL2MDP脂质体组为(4.3±1.1)个(P<0.05);流式细胞仪检测分离后肝脏贴壁细胞,PBS组细胞高表达CD11c(82.3±1.8)%,而CL2MDP脂质体为(7.3±0.9)%(P<0.01)。结论:本研究建立了CL2MDP脂质体剔除小鼠肝脏Kupffer细胞的模型,为进一步研究Kupffer细胞功能,提供新的手段。

AIM： To establish methods for preparing Dichloromethylenediphosphonic acid disodium salt liposomes（ CL2MDP）,and observe its elimination effect for kupffer cells in mouse liver METHODS： The liposome was used to parcel CL2 MDP.Spectrophotometer was used to detect encapsulation efficiency. The CL2 MDP liposomes was injected into the caudal vein of BALB / c mice（ 0. 1 m L /10 g）,after 3 days,the immunohistochemical method was used to detect the number of Kupffer cells in the liver of mice（ anti-mouse F4 /80 expression positive）.The expression of CD11 c in liver adherent cells from separated liver was examined by flow cytometry.RESULTS： The entrapment efficiency of CL2 MDP was（ 20. 1 ± 2. 9）. The immunohistochemical results showed that the number of kupffer cells of mice respectively were（ 16. 2 ± 1. 8） in PBS group and（ 4. 3 ± 1. 1） in CL2 MDP group（ P〈0. 05）. The expression of CD11 c was higher in PBS group（ 82. 3± 1. 8） % than CL2 MDP group（ 7. 3 ± 0. 9） %（ P〈0. 01）. CONCLUSION： This study establishes the mouse model that CL2 MDP liposomes knockout liver kupffer cells and provides new means to further research the function of kupffer cells.