摘要
目的观察和比较致敏小鼠及对照小鼠T细胞中T-bet、GATA3两种转录因子mRNA和蛋白表达的差异及大剂量过敏原在体外的作用。方法复制致敏小鼠模型,分离脾脏树突状细胞(Dc)及T细胞。反转录一聚合酶链反应(RT—PCR)和Western blotting法分别检测致敏小鼠和对照小鼠T细胞转录因子T—bet和GATA3的mRNA及蛋白表达水平。致敏小鼠脾脏DC、T细胞与10g/LOVA或生理盐水共培养后,RT-PCR和Western blotting法分别检测T—bet和GATA3的mRNA及蛋白表达。结果致敏小鼠T细胞T-bet的mRNA及蛋白表达较正常对照小鼠显著降低,而GATA3的表达较对照小鼠显著升高。大剂量过敏原(10g/L的OVA)作用下致敏小鼠T细胞T-bet的mRNA和蛋白表达水平较对照组显著降低,GATA3的mRNA和蛋白表达水平较对照组显著降低。结论致敏小鼠T细胞转录因子T-bet表达减少而GATA3表达增加,大剂量过敏原在体外诱导致敏小鼠T细胞T—bet和GATA3表达减少,其机制可能与诱导调节性T细胞极化等有关。
Objective To observe the effect of high dose allergen on the mRNA and protein levels of T-bet and GATA3. Methods The mRNA and protein levels of T-bet and GATA3 were detected by RT-PCR and Western blotting in allergic mice and normal mice. And the effect of OVA at 10 g/L was observed. Results The mRNA and protein levels of T-bet were lower in allergic mice than normal mice. And the mRNA and protein levels of GATA3 were higher in allergic mice than in normal mice. The mRNA and protein levels of T-bet and GATA3 were significantly decreased in T cells when treated by OVA at 10 g/L in allergic mice. Conclusions The expression of T-bet and GATA3 were suppressed by high dose allergen in allergic mice in vitro. And the underlying mechanism may be related to the regulatory T cells.
出处
《国际呼吸杂志》
2015年第17期1285-1288,共4页
International Journal of Respiration