狼疮性肾炎患者外周血中调节性T细胞与NKT细胞变化的研究

Patients with lupus nephritis regulatory cells and NKT cells in peripheral blood of change research

目的观察狼疮性肾炎（LN）患者外周血中的CIM＋CD25＋调节性T细胞（Tregs）、NK／NKT细胞变化规律及其与LN的关系，并探讨其意义。方法选择系统性红斑狼疮患者（无肾脏受累）17例，狼疮性肾炎患者23例，健康志愿者24例，分别做为SLE组、LN组和对照组。应用流式细胞仪检测其外周血中CD4＋CD25＋Tregs、CD3＋CD57＋NKT细胞、CD3-CD57＋NK＋细胞、CD3＋CD56＋NKT细胞、CD3-CD56＋NKT细胞、CD3．CD20＋B细胞，并且对狼疮性肾炎患者进行肾脏活检以及尿蛋白、抗核抗体等检测。结果LN组外周血中CD4＋CD25＋Tregs比例显著低于对照组（t=1．99，P=0．03），CD3＋CD57＋NKT细胞群比例显著高于对照组（t=1．98，P=0．02）；CD3一CD57＋NKT细胞、CD3＋CD56＋NKT细胞、CD3-CD56＋NKT细胞、CD3-CD20＋B细胞变化不明显。结论CD4＋CD25＋Tregs是阻止LN发生和发展的关键，CD3＋CD57＋NKT细胞对LN患者的。肾脏受损起重要作用。

Objective To investigate the ratios of CD4 ＋ CD25 ＋ regulatory cells （Tregs） and NK/NKT cells in peripheral blood of patients of lupus nephritis and to explore their contribution on the pathogenesis of lupus nephritis. Methods Patients of systemic lupus erythematosus （SLE） with no renal involvement,lupus nephritis and healthy controls were studied. Flow cytometry was utilized to quantify the percentage of CD4 ＋ CD25 ＋ Tregs, CD3 ＋ CD57 ＋ NKT cells, CD3 - CD57 ＋ NK, CD3 ＋ CD56 ＋ NKT cells, CD3 - CD56 ＋ NK cells and CD3 - CD20 ＋B cells in peripheral blood. Renal biopsy was performed for patients with lupus nephritis. Urine protein and antinuclear antibody were also detected. Results The mean percentage of CD4 ＋ CD25 ＋ Tregs in the pe- ripheral blood of patients with lupus nephritis was significantly lower than the control group （t = 1.99, P =0. 03） ,while CD3 ＋ CD57 ＋ NKT from lupus nephritis was significantly higher compared with control （ t = 1. 98, P = 0. 02）. However, levels of CD3 - CD57 ＋ NKT cells, CD3 ＋ CD56 ＋ NKT cells, CD3 - CD56 ＋ NKT cells and CD3-CD20 ＋ B cells did not show a significant change. Conclusions CIM ＋ CD25 ＋ Tregs may play an cru- cial role to prevent the occurrence and progression of lupus nephritis, while CD3 ＋ CD57 ＋ NKT cells in the peripheral blood from patients with lupus nephritis may exert an important effect on kidney damage.