消痰散结方对胃癌微血管密度及VEGF-A/VEGFR-2的影响

Impacts of Xiaotan Sanjie Formula on Microvessel Density and VEGF-A/VEGFR-2 in Gastric Cancer

目的研究消痰散结方对胃癌MVD及VEGF-A/VEGFR-2表达的影响。方法对20例自愿进行单纯中药抗肿瘤治疗的胃癌患者,留取消痰散结方进行干预前及干预6个月后的胃癌(癌组织、癌旁近端、远端)标本。采用免疫组化SP法检测CD34标记微血管密度MVD,Western Blot、RT-PCR检测VEGF-A/VEGFR-2的表达。结果消痰散结方对胃癌组、癌旁近端组、远端组微血管密度MVD和VEGF-A/VEGFR-2有不同程度的下调作用。胃癌组消痰散结方治疗后微血管密度和VEGF-A/VEGFR-2下降幅度最大(P<0.05),有统计学意义;近端组、远端组治疗后微血管密度和VEGF-A/VEGFR-2略有下降,无统计学意义(P>0.05)。结论消痰散结方治疗胃癌的部分作用机制可能与其抑制VEGF-A/VEGFR-2信号通路降低微血管密度有关。

Objective To study the impacts of xiaotan sanjie formula on microvessel density（ MVD）in VEGF- A / VEGFR- 2 expression in gastric cancer. Methods The samples of gastric cancer（ cancer tissue,proximal tissue,distal tissue） were collected before and 6 months after intervention with xiaotan sanjie formula in 20 volunteers treated with simple Chinese medicine for anti- tumor. IHC SP method was used to detect CD34 labeled MVD,western blot and RT- PCR methods were applied to detect VEGF- A / VEGFR-2 expression. Results Xiaotan sanjie formula down- regulates MVD and VEGF- A / VEGFR- 2 in the gastric cancer group,proximal end group and distal end group to different degrees. In the gastric cancer group,after the treatment with xiaotan sanjie formula,MVD and VEGF- A / VEGFR- 2 were reduced to the largest extent in the gastric cancer group（ P〈 0. 05）,indicating the statistical significance. In the proximal end group and distal end group,MVD and VEGF- A / VEGFR- 2 were reduced slightly（ P〉 0. 05）,without statistical significance. Conclusion The mechanism on a part of effects of xiaotan sanjie formula on gastric cancer is possibly related with inhibiting VEGF- A / VEGFR- 2 signal path and reducing MVD.