中药复方对ApoE基因敲除小鼠动脉粥样硬化不稳定斑块的影响及机制

Effect and Mechanism of Chinese Herbal Compound on ApoE Knockout Mice Animal Models of Unstable Plaque Atherosclerosis

目的 观察搜风祛痰中药复方稳斑汤对ApoE基因敲除小鼠动脉粥样硬化（AS）不稳定斑块的作用及机制。方法 6～8周龄ApoE基因缺陷鼠,高脂饲料喂养13周后造出AS不稳定斑块模型,同时将正常C57BL/6J小鼠10只正常饲料喂养13周,设为空白对照组。各组分别给予相应药物干预1个月,麻醉处死小鼠取主动脉组织HE染色后在光学显微镜下进行病理学观察,然后采用免疫组化和RT-PCR技术检测HSP70和PPAR-γ的表达水平。结果 与空白对照组比较,模型对照组HSP70和PPAR-γ明显下降（P〈0.01）;与模型对照组比较,中药低、中剂量组HSP70和PPAR-γ明显升增高（P〈0.05）,中药高剂量组与西药组较模型对照组明显增高（P〈0.01）,然而两者表达水平在中药高剂量组与西药组无明显差异（P〉0.05）。结论 ApoE基因敲除小鼠AS不稳定斑块的形成与炎症因子密切相关,搜风祛痰中药复方稳斑汤可能通过调节HSP70和PPAR-γ的表达而干预AS斑块形成及破裂,为AS的防治开辟了一条新思路。

Objective To observe the effect and mechanism of Chinese herbal compound on ApoE KnocKout mice animal models of unstable plaque AS. Methods Six to eight weeKs old ApoE gene - deficient mice were fed with high fat diet for 13 weeKs to establish atherosclerosis（atherosclerosis,AS）unstable plaque animal models,while 10 normal C57BL/ 6J mice were fed with normal diet for 13 weeKs to establish as the blanK control. Then they were given low dose,medium - dose,high - dose of Chinese herbal compound and western medicine for a month. At the same time,the model group was fed by normal sodium for one month. Mice were executed and the anesthetic and fetch aortic tissue were then observed under an optical microscope pathol-ogy after HE staining. Immunohistochemistry and RT - PCR technique were used to detect the expression levels of HSP70 and PPAR -γ. Results Compared with the control group,the expression level of HSP70 and PPAR- γ in model control group decreased significantly（P ﹤0. 01）;compared with the model control group,the ex-pression level of HSP70 and PPAR - γ in Chinese herbal compound low dose group significantly increased（P ﹤0. 05）;compared with the model control group,the expression level in Chinese herbal compound high - dose group and western medicine atorvastatin group was significantly higher（P ﹤0. 01）;however,the expression lev-els in the both two group had no significant difference（P ﹥0. 05）. Conclusion ApoE KnocKout mice AS un-stable plaque formation is closely related to inflammatory cytoKines. Searching wind and expectorant phlegm tra-ditional Chinese medicine may stabilize plaques by regulating the expression of HSP70 and PPAR -γ and pro-moting intervention on the formation and rupture of AS plaque,which provides a new idea to prevent AS.