摘要
目的观察坐珠达西对慢性萎缩性胃炎(CAG)模型大鼠血清丙二醛(MDA)、一氧化氮(NO)及白细胞介素-12(IL-12)的影响,探讨其治疗慢性萎缩性胃炎的作用机制。方法受试动物分别分为空白组10只及模型组70只,雌雄各半。模型组动物以氨水复合多因素造模12周,制备CAG模型。造模12周后,随机取模型组大鼠雌雄各5只,麻醉,解剖,取胃窦部做HE染色,观察组织病理学改变。模型组大鼠随机分为模型组、摩罗丹组、坐珠达西大、中、小剂量组,每组10只,雌雄各半。给药3个月后,取材。HE染色检查胃黏膜组织病理改变,试剂盒检测血清中MDA、NO及IL-12水平。结果与模型组比较,坐珠达西各剂量组及摩罗丹组大鼠血清中MDA含量下降(P<0.01,P<0.05)。与空白组比较,模型组大鼠血清中NO含量有所降低,但差异无统计学意义;与模型组比较,坐珠达西各剂量组大鼠血清中NO含量有所升高,但差异无统计学意义;坐珠达西小剂量组NO含量接近空白组。与模型组比较,坐珠达西大、中剂量组及摩罗丹组血清中IL-12含量显著降低(P<0.05)。结论坐珠达西有效治疗GAG模型大鼠,可能是通过改善血清MDA、IL-12含量,升高NO含量。
Objective To observe the effects of Zuozhu - Daxi Pill on chronic atrophic gastritis （chronic atrophic gastritis,CAG）model rats serum in terms of MDA,NO and IL - 12,and to explore its mechanism of action of the treatment of chronic atrophic gastritis. Methods All experiment animals were di-vided into blanK control group and model group. Model group animals employ ammonia complex multi - factor building for 12 weeKs to induce chronic atrophic gastritis（the CAG）model. Model rats were randomly divided into model control group,MoLuoDan group,Zuozhu - Daxi Pill large,medium and small dose group. Each group received intragastric administration one time every day. Three months later,drew materials needed for the study from the rats. Observe gastric mucosal tissue of pathological changes with HE staining,and MDA, NO and IL - 12 in serum. Results Compared with model group,each of the Zuozhu - Daxi Pill dose group of gastric tissue pathological forms have different degrees of improvement. MDA content in serum in each dose group decreased significantly（P ﹤ 0. 05）;large and medium dose group increased significantly. NO content in serum and IL - 12 levels decreased significantly（P ﹤ 0. 05）. Conclusion Zuozhu - Daxi Pill＇s effectiveness in treating GAG model rats may have happened through improving the serum MDA,IL - 12 levels,and in-creasing the NO content.
出处
《世界中西医结合杂志》
2015年第5期636-638,644,共4页
World Journal of Integrated Traditional and Western Medicine
基金
藏医药产业技术创新服务平台(2013-488)
