尾加压素Ⅱ在妊娠期糖尿病孕妇血清及胎盘中的表达及意义

Expressions and significance of urotensin Ⅱ in serum and placenta of pregnant women with gestational diabetes mellitus

目的探讨正常孕妇和妊娠期糖尿病（GDM）孕妇血清和胎盘中尾加压素Ⅱ（UⅡ）的表达及其临床意义。方法选取2012年6月-2013年12月在该院正规产检并住院分娩的孕妇80例,其中正常孕妇40例（对照组）,GDM孕妇40例（观察组）。采用酶联免疫吸附试验（ELISA）法检测两组孕妇外周血血清中的UⅡ水平;采用RT-PCR观察胎盘中UⅡmRNA的表达。结果两组孕妇血清中UⅡ水平值比较,差异有统计学意义（t=2.32,P〈0.05）;两组孕妇胎盘组织中UⅡmRNA表达水平比较,差异有统计学意义（t=1.67,P〈0.05）;两组血清UⅡ水平与胎盘组织UⅡmRNA表达水平均呈正相关（r=0.73,P〈0.05;r=0.72,P〈0.05）。结论 UⅡ在GDM孕妇血清及胎盘中的高表达提示其参与了GDM的发生与发展。

Objective To explore the expressions and clinical significance of urotensin Ⅱ（ UⅡ） in serum and placenta of normal pregnant women and pregnant women with gestational diabetes mellitus（ GDM）. Methods Eighty hospitalized pregnant women receiving prenatal examination in the hospital from June 2012 to December 2013 were selected and divided into control group（ 40 normal pregnant women） and observation group（ 40 GDM pregnant women）. ELISA was used to detect serum UⅡ levels in peripheral blood of women in the two groups; RT- PCR was used to observe the expression of UⅡ mRNA in placenta. Results There was statistically significant difference in serum UⅡ level between the two groups（ t = 2. 32,P 〈0. 05）; there was statistically significant difference in expression level of UⅡ mRNA in placenta between the two groups（ t = 1. 67,P 〈0. 05）; in the two groups,there were positive correlations between serum UⅡ levels and expression levels of UⅡ mRNA in placenta（ r = 0. 73,P 〈0. 05; r = 0. 72,P 〈0. 05）. Conclusion High expressions of UⅡ in serum and placenta of GDM women are related to occurrence and development of GDM.